Active Recombinant Human IL25 protein, His-tagged

• Cat.No.: IL25-1031H
• Product Overview: Recombinant Human IL25 protein(Tyr33-Gly177), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 33-177 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is 5.46-21.84ng/mL, corresponding to a specific activity of 4.58×10^4~1.83×10^5 units/mg.
• Molecular Mass: 17.58 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: IL25 interleukin 25 [ Homo sapiens ]
• Official Symbol: IL25
• Synonyms: IL25; interleukin 25; IL17E, interleukin 17E; interleukin-25; IL 17E; IL 25; interleukin 17E; interleukin-17E; IL17E
• Gene ID: 64806
• mRNA Refseq: NM_022789
• Protein Refseq: NP_073626
• MIM: 605658
• UniProt ID: Q9H293