Active Recombinant Full Length Human PFN1 Protein, C-Flag-tagged

• Cat.No.: PFN1-624HFL
• Product Overview: Recombinant Full Length Human PFN1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the profilin family of small actin-binding proteins. The encoded protein plays an important role in actin dynamics by regulating actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome, and the encoded protein may also play a role in Huntington disease. Multiple pseudogenes of this gene are located on chromosome 1.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Pull-down assay
• Molecular Mass: 14.9 kDa
• AA Sequence: MAGWNAYIDNLMADGTCQDAAIVGY KDSPSVWAAVPGKTFVNITPAEVGV LVGKDRSSFYVNGLTLGGQK CSVIRDSLLQDGEFSMDLRTKSTGG APTFNVTVTKTDKTLVLLMGKEGVH GGLINKKCYEMASHLRRSQYTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Regulation of actin cytoskeleton

Gene Information

• Gene Name: PFN1 profilin 1 [ Homo sapiens (human) ]
• Official Symbol: PFN1
• Synonyms: ALS18
• Gene ID: 5216
• mRNA Refseq: NM_005022.4
• Protein Refseq: NP_005013.1
• MIM: 176610
• UniProt ID: P07737

Reviews

08/26/2021

The manufacturer ensures that the protein is produced with utmost purity and integrity, minimizing the risk of experimental artifacts or compromised results.

06/22/2021

Their excellent technical support, commitment to scientific collaboration, and efficient supply chain management greatly contribute to the success and productivity of my research.

11/09/2020

One of the key advantages of using PFN1 protein in trials is its high quality.

Q&As

Is PFN1 protein involved in any other cellular processes apart from cell motility?

Yes, PFN1 is involved in processes such as endocytosis, cytokinesis, and cell signaling, highlighting its importance in various cellular functions.

Are there ongoing clinical trials targeting PFN1 for cancer treatment?

Research on targeting PFN1 in cancer therapy is ongoing, and there are clinical trials exploring potential drugs that could inhibit PFN1 activity to impede cancer cell metastasis.

How does PFN1 protein contribute to cancer progression?

PFN1 has been found to promote cancer cell migration and invasion, suggesting its role in cancer metastasis, making it a potential target for cancer therapy.

Can PFN1 protein be targeted for drug development?

Yes, researchers are exploring the potential of developing drugs that target PFN1 to modulate its activity, especially in the context of cancer metastasis and neurodegenerative disorders.

How does PFN1 protein affect cell motility and migration?

PFN1 regulates cell motility and migration by controlling the dynamics of actin filaments, essential components of the cell's cytoskeleton involved in cell movement.