||Recombinant Human PFN1 was produced in E. coli.
||Profilin (15kDa) regulates the G-actin pool by sequestration of actin monomers. Thus profilin controls the growth of actin filaments, and is thus an essential regulator of cellular locomotion and cell shape changes.
Profilin is negatively regulated by phospholipids (PIP2, InsP3), reversed by phospholipase C.
||150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 50µl ultrapure water to obtain a 1.0 mg/ml solution.
||>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
||Profilin is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20 centigrade in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles.
||At ambient temperature. Upon delivery store at -70 centigrade.