| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
1-140 aa |
| Description : |
Alpha-synuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. Abnormalities in alpha-synuclein are implicated in the pathogenesis of Parkinson's disease (PD). Alpha-synuclein is present in Lewy-bodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration. |
| Form : |
Lyophilized from 0.22 μm filtered solution in 100 mM NaAC, pH7.0 with trehalose as protectant. |
| Bio-activity : |
Immobilized Human Alpha-Synuclein (A53T), His Tag at 1 μg/mL (100 μL/well) can bind Synuclein alpha/beta Monoclonal Antibody with a linear range of 1-31 ng/mL. |
| Molecular Mass : |
The protein has a calculated MW of 16.4 kDa. The protein migrates as 18-19 kDa under reducing (R) condition (SDS-PAGE). |
| Purity : |
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS. |
| Storage : |
This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
