Species : |
Human |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
19-1676 a.a. |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Human Complement C5 (w917s), His Tag at 2 μg/mL (100 μL/well) can bind Anti-C5a MAb (Human IgG1) with a linear range of 0.2-6 ng/mL.Immobilized Human Complement C5 (w917s), His Tag at 2 μg/mL (100 μL/well) can bind Eculizumab Biosimilar with a linear range of 0.004-0.25 μg/mL. |
Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.3 kDa (α chain). The protein migrates as 80 kDa (β chain) and 110 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : |
Gln19-Cys1676 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. |
Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |