Active Recombinant Human CTSD protein, His-tagged

• Cat.No.: CTSD-04H
• Product Overview: Recombinant Human CTSD protein, fused to His-tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 21-412aa
• Description: This gene encodes a member of the A1 family of peptidases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease.
• Form: Liquid in. 50mM MES buffer (pH 5.5) containing 100mM NaCl, 20% glycerol.
• Bio-activity: Specific activity is > 20 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 3.5 at 25 centigrade.
• Molecular Mass: 43.4 kDa
• AA Sequence: LVRIPLHKFT SIRRTMSEVG GSVEDLIAKG PVSKYSQAVP AVTEGPIPEV LKNYMDAQYY GEIGIGTPPQ CFTVVFDTGS SNLWVPSIHC KLLDIACWIH HKYNSDKSST YVKNGTSFDI HYGSGSLSGY LSQDTVSVPC QSASSASALG GVKVERQVFG EATKQPGITF IAAKFDGILG MAYPRISVNN VLPVFDNLMQ QKLVDQNIFS FYLSRDPDAQ PGGELMLGGT DSKYYKGSLS YLNVTRKAYW QVHLDQVEVA SGLTLCKEGC EAIVDTGTSL MVGPVDEVRE LQKAIGAVPL IQGEYMIPCE KVSTLPAITL KLGGKGYKLS PEDYTLKVSQ AGKTLCLSGF MGMDIPPPSG PLWILGDVFI GRYYTVFDRD NNRVGFAEAA RL
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
• Purity: > 95%
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: CTSD cathepsin D [ Homo sapiens (human) ]
• Official Symbol: CTSD
• Synonyms: CPSD; CTSD; Cathepsin D; CLN10; HEL-S-130P; lysosomal aspartyl protease; lysosomal aspartyl peptidase; lysosomal aspartyl protease
• Gene ID: 1509
• mRNA Refseq: NM_001909
• Protein Refseq: NP_001900
• MIM: 116840
• UniProt ID: P07339