| Species : |
Human |
| Source : |
E.coli |
| Tag : |
Non |
| Protein Length : |
27-207 aa |
| Form : |
Lyophilized from a 0.2µm filtered concentrated solution in 20mMPB pH7.2, with NaCL and Trehalose as protectant. |
| Bio-activity : |
Fully biologically active when compared to standard. Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 10ng/ml in the presence of 1µg/ml heparin, corresponding to a specific activity of ≥ 1 x 10^5 units/mg. |
| Molecular Mass : |
Approximately 21.0kDa, a single non-glycosylated polypeptide chain containing 181 amino acids. |
| AASequence : |
AEENVDFRIHVENQTRARDDVSRKQLRLYQLYSRTSGKHIQVLGRRISARGEDGDKYAQLLVETDTFGSQVRIKGKETEFYLCMNRKGKLVGKPDGTSKECVFIEKVLENNYTALMSAKYSGWYVGFTKKGRPRKGPKTRENQQDVHFMKRYPKGQPELQKPFKYTTVTKRSRRIRPTHPA |
| Endotoxin : |
<0.01EU/μg rHuFGF-18 protein as determined by LAL method. |
| Purity : |
>96% by SDS-PAGE or HPLC. |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |