Active Recombinant Human FLT3LG Protein, His-tagged

• Cat.No.: FLT3LG-4374H
• Product Overview: Human FLT3LG (156 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).

Specification

• Species: Human
• Source: Barley Grain
• Tag: His
• Description: Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011]
• Form: Lyophilized
• Bio-activity: The ED50 in the range of 0.12-0.18 ng/mL, corresponding to specific activity 6.62 X 106 U/mg. We recommend that the optimal concentration for each specific aplication be determined by an initial dose-response assay.
• Molecular Mass: 20, 22 kDa
• Endotoxin: Endotoxin level is less than 0.005 ng per μg protein (0.05EU/μg) as measured by turbidimetric kinetic assay.
• Purity: > 98% by SDS-PAGE
• Applications: Western Blot (Recombinant protein)
  Functional Study
  SDS-PAGE
• Storage: Store at -20 centigrade on dry atmosphere.After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20 centigrade. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Aliquot to avoid repeated freezing and thawing.
• Concentration: 100 μg/mL
• Storage Buffer: Lyophilized from 10 mM acetic acid, pH 5.0

Gene Information

• Gene Name: FLT3LG fms-related tyrosine kinase 3 ligand [ Homo sapiens ]
• Official Symbol: FLT3LG
• Synonyms: FLT3LG; fms-related tyrosine kinase 3 ligand; flt3 ligand; FL; FLT3L
• Gene ID: 2323
• mRNA Refseq: NM_001204502
• Protein Refseq: NP_001191431
• MIM: 600007
• UniProt ID: P49771