Active Recombinant Human IL13 Protein (Gly35-Asn146), C-His tagged, Animal-free, Carrier-free

Cat.No. : IL13-179H
Product Overview : Recombinant Human IL13 Protein (Gly35-Asn146) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.
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  • Gene Information
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Description : Interleukin 13 (IL-13) with a molecular mass of 10 kDa cytokine, it secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. It is homologous to IL-4 and shares many of its biologic activities on mononuclear phagocytic cells, endothelial cells, epithelial cells, and B cells.
Source : E. coli
Species : Human
Tag : C-His
Form : Lyophilized
Bio-activity : Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.8 ng/mL. The specific activity of recombinant human IL-13 is approximately >1 × 10⁶ IU/ mg.
Molecular Mass : The protein has a calculated MW of 13.28 kDa.
The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).
Protein Length : Gly35-Asn146
Endotoxin : < 0.01 EU/μg of the protein by the LAL method.
Purity : >95% as determined by SDS-PAGE analysis.
Storage : This product is stable after storage at:
-20 centigrade for 12 months in lyophilized state from date of receipt.
-20 or -80 centigrade for 2 weeks under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Storage Buffer : Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping : The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.
Gene Name : IL13 interleukin 13 [ Homo sapiens (human) ]
Official Symbol : IL13
Synonyms : IL13; interleukin 13; interleukin-13; allergic rhinitis; ALRH; BHR1; Bronchial hyperresponsiveness 1 (bronchial asthma); IL 13; MGC116786; MGC116788; MGC116789; P600; Bronchial hyperresponsiveness-1 (bronchial asthma); IL-13;
Gene ID : 3596
mRNA Refseq : NM_002188
Protein Refseq : NP_002179
MIM : 147683
UniProt ID : P35225

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How does flow cytometry help quantify IL13 receptor expression on different cell populations? 12/29/2022

Flow cytometry quantifies IL13 receptor expression on cells.

What are the benefits of using transgenic animal models to study IL13's in vivo functions? 07/05/2022

Transgenic models illuminate IL13's in vivo functions.

Could you explain the role of ELISA in quantifying IL13 secretion levels in cell cultures? 02/23/2022

ELISA quantifies IL13 secretion in cell cultures accurately.

How can siRNA knockdown experiments reveal IL13's effects on specific cellular processes? 06/13/2021

siRNA knockdown uncovers IL13's specific cellular effects.

How does co-immunoprecipitation identify interacting partners in IL13-mediated signaling complexes? 07/28/2019

Co-immunoprecipitation identifies IL13 signaling partners.

What techniques can differentiate IL13-mediated signaling pathways from IL4 pathways? 07/23/2019

Differential techniques distinguish IL13 and IL4 signaling pathways.

Could you elaborate on the use of reporter gene assays to assess IL13-driven transcriptional activity? 12/16/2018

Reporter gene assays gauge IL13-driven transcription.

Can you describe the applications of microarray analysis in profiling IL13-induced gene expression changes? 11/14/2018

Microarray analysis profiles IL13-induced gene expression.

What experimental methods determine the impact of IL13 on tissue remodeling processes? 08/04/2018

Experimental methods reveal IL13's impact on tissue remodeling.

How does site-directed mutagenesis help identify critical residues in IL13 receptor binding? 07/04/2018

Site-directed mutagenesis identifies key residues in IL13 receptor binding.

Customer Reviews (3)

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Reviews
11/29/2021

    Effective in Th2 cell differentiation.

    04/22/2020

      Good for cytokine assays.

      01/26/2019

        High yield in expression.

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