Active Recombinant Human IL17A Protein (136 aa)

• Cat.No.: IL17A-100I
• Product Overview: Recombinant Human IL17A Protein without tag was expressed in E. coli.

Specification

• Description: Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-κB and costimulation of T cell proliferation.
• Source: E. coli
• Species: Human
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent induction of IL-6 in primary human foreskin fibroblasts was found to be approximately 2 ng/mL, corresponding to a specific activity of > 5 × 10^5 units/mg.
• Molecular Mass: Approximately 31.0 kDa, disulfide-linked homodimer of two 136 amino acid polypeptide chains.
• Protein length: 136
• AA Sequence: MIVKAGITIPRNPGCPNSEDKNFPR TVMVNLNIHNRNTNTNPKRSSDYYN RSTSPWNLHRNEDPERYPSVIWEAK CRHLGCINADGNVDYHMNSVPIQQE ILVLRREPPHCPNSFRLEKILVSVG CTCVTPIVHHVA
• Endotoxin: Less than 1 EU/mg of rHuIL-17 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: IL17A interleukin 17A [ Homo sapiens ]
• Official Symbol: IL17A
• Synonyms: IL17A; interleukin 17A; CTLA8, IL17, interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8); interleukin-17A; cytotoxic T lymphocyte associated protein 8; IL 17; IL 17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; cytotoxic T-lymphocyte-associated serine esterase 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); IL17; CTLA8; IL-17; IL-17A
• Gene ID: 3605
• mRNA Refseq: NM_002190
• Protein Refseq: NP_002181
• MIM: 603149
• UniProt ID: Q16552

Reviews

12/16/2018

Good for receptor studies.

03/05/2018

Effective in Th17 differentiation.

03/08/2017

High bioactivity in various tissue types.

Q&As

Could you explain the role of IL17A in autoimmune disease progression using animal models?

Animal models, such as experimental autoimmune encephalomyelitis, explore IL17A's role in autoimmunity.

Can you detail the protocols for inducing IL17A expression in T cells in vitro?

Inducing IL17A expression in T cells involves culturing them with specific cytokines.

What strategies elucidate IL17A's impact on tissue-specific inflammation in human samples?

Studying IL17A's influence on tissue-specific inflammation in humans involves analyzing patient samples.

Can you elaborate on the use of neutralizing antibodies in blocking IL17A's effects?

Neutralizing antibodies block IL17A's effects in cell cultures or animal models.

How does one perform a multiplex cytokine assay to quantify IL17A along with other cytokines?

Multiplex cytokine assays use fluorescent beads to measure IL17A and other cytokines simultaneously.

How is the IL17A receptor (IL17RA) signaling pathway activated and studied?

IL17RA signaling is triggered by IL17A binding and is studied through downstream phosphorylation assays.

What are the methods to investigate the impact of IL17A on epithelial barrier integrity?

Methods like transepithelial electrical resistance (TEER) assay evaluate IL17A's effect on barrier function.

How is IL17A's contribution to neutrophil recruitment assessed in inflammatory models?

Inflammatory models, like airway inflammation assays, examine IL17A's impact on neutrophil recruitment.

What techniques are used to analyze IL17A-producing cell populations within a tissue?

Flow cytometry or intracellular staining help identify IL17A-producing cell subsets within tissues.

What experimental design is employed to understand IL17A's involvement in cancer development?

Experimental designs combine cancer cell lines with IL17A stimulation to investigate its pro-tumor effects.