Active Recombinant Canine interleukin 17A protein, His tagged

• Cat.No.: IL17A-12C
• Product Overview: Recombinant canine IL-17A (29-155 aa), fused to Histag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene is a member of the IL-17 receptor family which includes five members (IL-17RA-E) and the encoded protein is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. The encoded protein elicits crucial impacts on host defense, cell trafficking, immune modulation, and tissue repair, with a key role in the induction of innate immune defenses. This cytokine stimulates non-hematopoietic cells and promotes chemokine production thereby attracting myeloid cells to inflammatory sites. This cytokine also regulates the activities of NF-kappaB and mitogen-activated protein kinases and can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). IL-17A plays a pivotal role in various infectious diseases, inflammatory and autoimmune disorders, and cancer. High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The lung damage induced by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL17A.
• Source: HEK293
• Species: Canine
• Tag: His
• Protein length: 29-155 aa
• Form: Liquid
• Bio-activity: The activity is determined by the IL-6 ELISA in a using NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 10 ng/mL.
• Molecular Mass: 15.6 kDa (133aa)
• AA Sequence: FPQNPGCRNTEDKNFPQHVKVNLNI LNRNTNSRRPSDYYNRSTSPWNLHR NEDPERYPSVIWEAKCRHLGCVNNE GNINYHMNSVPIQQEILVLRRESQH CPHSFRLEKMLVAVGCTCVTPIVRH VA
• Endotoxin: < 1 EU/μg by LAL
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Storage Buffer: In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol.
• Concentration: 0.5 mg/mL (determined by Absorbance at 280nm)
• Reference: 1. Moseley TA., et al, (2003) Cytokine & Growth Factor Reviews. 14: 155-174.
  2. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32-46.
  3. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593-2603.

Gene Information

• Gene Name: IL17A interleukin 17A [ Canis lupus familiaris (dog) ]
• Official Symbol: IL17A
• Synonyms: IL17A; interleukin 17A; interleukin-17A
• Gene ID: 481837
• mRNA Refseq: NM_001165878
• Protein Refseq: NP_001159350
• UniProt ID: C6L8D7

Reviews

12/16/2018

Good for receptor studies.

03/05/2018

Effective in Th17 differentiation.

03/08/2017

High bioactivity in various tissue types.

Q&As

Could you explain the role of IL17A in autoimmune disease progression using animal models?

Animal models, such as experimental autoimmune encephalomyelitis, explore IL17A's role in autoimmunity.

Can you detail the protocols for inducing IL17A expression in T cells in vitro?

Inducing IL17A expression in T cells involves culturing them with specific cytokines.

What strategies elucidate IL17A's impact on tissue-specific inflammation in human samples?

Studying IL17A's influence on tissue-specific inflammation in humans involves analyzing patient samples.

Can you elaborate on the use of neutralizing antibodies in blocking IL17A's effects?

Neutralizing antibodies block IL17A's effects in cell cultures or animal models.

How does one perform a multiplex cytokine assay to quantify IL17A along with other cytokines?

Multiplex cytokine assays use fluorescent beads to measure IL17A and other cytokines simultaneously.

How is the IL17A receptor (IL17RA) signaling pathway activated and studied?

IL17RA signaling is triggered by IL17A binding and is studied through downstream phosphorylation assays.

What are the methods to investigate the impact of IL17A on epithelial barrier integrity?

Methods like transepithelial electrical resistance (TEER) assay evaluate IL17A's effect on barrier function.

How is IL17A's contribution to neutrophil recruitment assessed in inflammatory models?

Inflammatory models, like airway inflammation assays, examine IL17A's impact on neutrophil recruitment.

What techniques are used to analyze IL17A-producing cell populations within a tissue?

Flow cytometry or intracellular staining help identify IL17A-producing cell subsets within tissues.

What experimental design is employed to understand IL17A's involvement in cancer development?

Experimental designs combine cancer cell lines with IL17A stimulation to investigate its pro-tumor effects.