Active Recombinant Human IL17C protein

• Cat.No.: IL17C-185H
• Product Overview: Recombinant Human IL17C(His19-Val197) fused with Met at N-terminal was expressed in E. coli.

Specification

• Description: Interleukin‑17C (IL‑17C) is a 15‑20 kDa glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold. Mature human IL‑17C shares 79% and 76% amino acid sequence identity with mouse and rat IL‑17C, respectively. IL‑17C binds to IL‑17 RE with high affinity and to IL‑17 RA with low affinity. These two receptor chains can associate into a heterodimeric receptor for IL‑17C. IL‑17 RE is expressed on keratinocytes, mucosal epithelial cells, Th17 cells, and gamma /δ T cells, while IL‑17 RA is widely expressed. IL‑17 RE is required for mediating the pro‑inflammatory and homeostatic actions of IL‑17C in the skin and mucosa. IL‑17C expression is induced by inflammatory stimulation in colon and airway epithelial cells, keratinocytes, CD4+ T cells, macrophages, and dendritic cells. It is up‑regulated in various chronic inflammatory diseases including psoriasis, cystic fibrosis, and chronic obstructive pulmonary disease (COPD). IL‑17 RE is reciprocally down‑regulated in psoriatic lesions (10). The interaction of IL‑17C with IL‑17 RE promotes mucosal immunity through the induction of anti‑bacterial peptides and pro‑inflammatory cytokines and chemokines. IL‑17C action supports the integrity of the colon epithelium following infection induced damage but also contributes to psoriatic skin thickening and the progression of arthritis. IL‑17C is additionally up‑regulated in Th17 cell dependent autoimmunity. In this setting, it exacerbates disease severity by inducing Th17 cell production of IL‑17A, IL‑17F, IL‑22, CCR6, and CCL20.
• Source: E. coli
• Species: Human
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human IL-17 RE Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human IL-17C that produces 50% of the optimal binding response is 1.5-7.5 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 19.8 (monomer) kDa
• Protein length: His19-Val197
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Tag: Non

Gene Information

• Gene Name: IL17C interleukin 17C [ Homo sapiens ]
• Official Symbol: IL17C
• Synonyms: IL17C; interleukin 17C; interleukin-17C; CX2; IL 17C; IL 21; MGC126884; MGC138401; cytokine CX2; IL-21; IL-17C
• Gene ID: 27189
• mRNA Refseq: NM_013278
• Protein Refseq: NP_037410
• MIM: 604628
• UniProt ID: Q9P0M4
• Chromosome Location: 16q24
• Function: cytokine activity

Reviews

11/26/2021

High purity levels.

03/26/2021

Potent in anti-fungal assays.

06/07/2020

Suitable for receptor binding studies.

Q&As

How is the involvement of IL17C in autoimmune diseases elucidated using experimental models and patient samples?

Animal models, patient biopsies, and genetic association studies reveal IL17C's role in autoimmune diseases.

Can you explain the experimental designs used to study the crosstalk between IL17C and other cytokines in inflammatory responses?

Combination cytokine stimulation assays and signaling pathway inhibitors elucidate IL17C's crosstalk.

How is the function of IL17C in promoting tumor growth and progression investigated in cancer models?

Xenograft models and tumor growth measurements uncover IL17C's contribution to cancer progression.

How are the effects of IL17C modulation on specific immune cell populations characterized using flow cytometry and cell sorting?

Flow cytometry analysis with cell-specific markers characterizes IL17C's impact on immune cell subsets.

What molecular techniques are utilized to validate the therapeutic potential of targeting IL17C in inflammatory disorders?

Neutralizing antibodies and RNA interference validate IL17C's potential as a therapeutic target.

What approaches are taken to assess the role of IL17C in mucosal immunity and host defense against infections?

Infection models, bacterial challenges, and mucosal sampling assess IL17C's role in host defense.

Could you elaborate on the methods used to study IL17C's contribution to tissue-specific immune cell recruitment?

Chemotaxis assays and confocal microscopy study IL17C-mediated immune cell recruitment.

What strategies are used to explore the impact of IL17C on epithelial barrier integrity and inflammation?

Organoid culture systems and cytokine measurements explore IL17C's impact on epithelial barrier function.

What experimental techniques are employed to investigate the binding partners of IL17C and its downstream signaling pathways?

Co-immunoprecipitation and mass spectrometry identify IL17C interactors and signaling molecules.

How do researchers differentiate the functional roles of IL17C from other IL17 family members in immune responses?

Comparative functional assays and gene knockout models distinguish IL17C's unique roles.