Active Recombinant Human IL25 protein, hFc-tagged
Cat.No. : | IL25-148H |
Product Overview : | Recombinant Human IL25 protein(NP_073626.1)(Tyr 33-Gly177), fused with hFc tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | hFc |
Form : | Lyophilized from sterile PBS , pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. Immobilized human IL17BR-His at 10 μg/ml (100 μl/well) can bind human Fc-IL25, The EC50 of human Fc-IL25 is 1.7-4.2 μg/mL.2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED50 for this effect is 15-60 ng/mL. |
Molecular Mass : | The recombinant human IL25/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 amino acids and predicts a molecular mass of 45 KDa. As a result of glycosylation, the apparent molecular mass of rh IL25/Fc monomer is approximately 50-60 KDa in SDS-PAGE under reducing conditions. |
Proteinlength : | Tyr33-Gly177 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | IL25 interleukin 25 [ Homo sapiens ] |
Official Symbol : | IL25 |
Synonyms : | IL25; interleukin 25; IL17E, interleukin 17E; interleukin-25; IL 17E; IL 25; interleukin 17E; interleukin-17E; IL17E; |
Gene ID : | 64806 |
mRNA Refseq : | NM_022789 |
Protein Refseq : | NP_073626 |
MIM : | 605658 |
UniProt ID : | Q9H293 |
Products Types
◆ Recombinant Protein | ||
IL25-1924M | Recombinant Mouse IL25 Protein | +Inquiry |
IL25-509H | Recombinant Human IL25 Protein | +Inquiry |
IL25-182H | Recombinant Active Human IL25 Protein, His-tagged(C-ter) | +Inquiry |
Il25-1010M | Active Recombinant Mouse Il25 Protein | +Inquiry |
IL25-1321C | Recombinant Canine IL25 protein(Leu17-Ala169), hFc-tagged | +Inquiry |
◆ Lysates | ||
IL25-2920HCL | Recombinant Human IL25 cell lysate | +Inquiry |
IL25-1261RCL | Recombinant Rat IL25 cell lysate | +Inquiry |
IL25-2910MCL | Recombinant Mouse IL25 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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