Active Recombinant Human Il27RA protein, hFc-tagged

• Cat.No.: Il27RA-1629H
• Product Overview: Active Recombinant Human Il27RA protein(Met 1-Arg 514)(NP_004834.1) was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-514 aa
• Form: Lyophilized from sterile PBS, pH 7.4, 8% trehalose, 8% mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human IL27 at 20 μg/ml (100 μl/well) can bind human IL27Ra with a linear ranger of 0.064-1.6 μg/ml.
• Molecular Mass: The recombinant human IL27Rα/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 722 amino acids and has a predicted molecular mass of 79 kDa. As a result of glycosylation, rh IL27Rα/Fc monomer migrates as an approximately 105-115 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL27RA interleukin 27 receptor, alpha [ Homo sapiens ]
• Official Symbol: Il27RA
• Synonyms: IL27RA; interleukin 27 receptor, alpha; interleukin-27 receptor subunit alpha; CRL1; IL 27R; T cell cytokine receptor type 1; TCCR; WSX 1; WSX1; zcytor1; IL-27RA; IL-27R-alpha; IL-27R subunit alpha; cytokine receptor-like 1; class I cytokine receptor; IL-27 receptor subunit alpha; T-cell cytokine receptor type 1; type I T-cell cytokine receptor; IL27R
• Gene ID: 9466
• mRNA Refseq: NM_004843
• Protein Refseq: NP_004834
• MIM: 605350
• UniProt ID: Q6UWB1