| Cat.No. : |
Il27ra-660M
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant Mouse Il27raprotein was expressed in Murine myeloma cell line with the Fc part of humanIgG1 Pro100-Lys330 (C-terminus) and Gly29-Lys510 (N-terminus). |
| Description : |
Il27ra (also knownas WSX1 and TCCR) is an 85-95 kDa member of the type I, group 2 cytokinereceptor family. Mature Il27ra is a type I transmembrane glycoprotein thatcontains a 486 amino acids extracellular region, a 21 aa transmembranesegment and a 92 aa cytoplasmic domain. Consistent with type I cytokinereceptors, the extracellular region contains four positionally conservedcysteine residues, a WSxWS motif (for receptor folding and ligand binding),and three fibronectin type III repeats. |
| Source : |
Mammalian cells |
| Form : |
Lyophilized from a0.2 μm filtered solution in PBS. |
| N-terminalSequence : |
Gly29 |
| Molecular Weight : |
80 kDa |
| Activity : |
Measuredby its ability to inhibit IL-27-antiviral activity using HepG2 humanhepatocellular carcinoma cells infected with encephalomyocarditis (EMC)virus. Bender H. et al. (2009) Hepatology 50:585. The ED50 forthis effect is typically 2-12 μg/mL. |
| Endotoxin Level : |
<1.0 EUper 1 μg of the protein by the LAL method. |
| SDS-PAGE : |
105-115kDa, reducing conditions |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstituteat 100 μg/mL in sterile PBS |
| Storage : |
Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt,-20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
