| Cat. No. : |
IL18-433H |
| Description : |
IL-18 is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. |
| Source : |
Human |
| Host : |
Escherichia Coli. |
| Form : |
Lyophilized from a concentrated (1mg/ml) solution in water containing 0.5x PBS pH=7.3. |
| Purity : |
Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Bio-activity : |
The ED50 as determined by the dose-dependant stimulation of the IFN-g by human PBMC co-stimulated with IL-12 was found to be 5ng/ml corresponding to a Specific Activity of 200,000IU/mg. |
| Physical Appearance : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Solubility : |
It is recommended to reconstitute the lyophilized Interleukin 18 in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage : |
Lyophilized Interleukin 18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Pathways : |
African trypanosomiasis; Cellular roles of Anthrax toxin; Cytokine Signaling in Immune system; Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway; Hypertrophy Model; IL12 signaling mediated by STAT4; IL12-mediated signaling events; Immune System; Influenza A; Interleukin-1 processing; Malaria; NOD-like receptor signaling pathway; Rheumatoid arthritis; Signaling by Interleukins; Tuberculosis |
