Active Recombinant Human L1CAM, Fc Chimera

• Cat.No.: L1CAM-485H
• Product Overview: Recombinant Human L1CAM (Accession # CAA42508) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Predicted N Terminal: Ile20 & Arg864
• Form: Lyophilized from a 0.2 µm filtered solution in Tris-Citrate and NaCl.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.When 5 x 10^4 cells/well are added to Recombinant Human L1CAM Fc Chimera coated plates (25 µg/mL with 100 µL/well), >30% will adhere for 1 hour incubation at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human L1CAM, Fc Chimera has a calculated MW of 150.4 kDa (monomer) & 55.9 kDa (truncated). In SDS-PAGE migrates as 210-225 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: L1CAM L1 cell adhesion molecule [ Homo sapiens ]
• Official Symbol: L1CAM
• Synonyms: L1CAM; L1 cell adhesion molecule; antigen identified by monoclonal R1 , HSAS, HSAS1, MASA, MIC5, S10, SPG1; neural cell adhesion molecule L1; CD171; antigen identified by monoclonal R1; S10; HSAS; MASA; MIC5; SPG1; CAML1; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• Gene ID: 3897
• mRNA Refseq: NM_000425
• Protein Refseq: NP_000416
• MIM: 308840
• UniProt ID: P32004
• Chromosome Location: Xq28