Active Recombinant Human MMP2 Protein (115-214, 393-447)

Cat.No. : MMP2-12H
Product Overview : Matrix Metalloproteinase-2 (MMP-2, Gelatinase A, Type IV collagenase) catalytic domain without fibronectin domains cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-2 fibronectin deficient (residues 115-214 and 393-447 where the residue numbers are based on the unprocessed precursor, UniProtKB accession P08253).
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Description : Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
Source : E. coli
Species : Human
Bio-activity : > 40 U/μg. Activity described as U=100 pmol/min at 25 centigrade using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 as substrate.
Molecular Mass : 17.5 kDa
Protein Length : 115-214, 393-447
AA Sequence : M-RKPKWDKNQITYRIIGYTPDLDPET VDDAFARAFQVWSDVTPLRFSRIHD GEADIMINFGRWEHGDGYPFDGKDG LLAHAFAPGTGVGGDSHFDDDELWT QGYSLFLVAAHEFGHAMGLEHSQDP GALMAPIYTYTKNFRLSQDDIKGIQ ELYGA
Purity : > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 14.4 and 18.4 kDa.
Applications : Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.
Storage : At -80 centigrade. After initial defrost, aliquot the product into individual tubes and refreeze at -80 centigrade. Avoid repeated freeze/thaw cycles.
Concentration : 0.15 mg/mL
Storage Buffer : Tris 20 mM pH 7.2, CaCl2 10 mM, ZnCl2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.5 M, glycerol 10%, Brij-35 0.05%. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280= 32430 M-1cm-1 calculated).
Shipping : On Dry Ice
Gene Name : MMP2 matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) [ Homo sapiens (human) ]
Official Symbol : MMP2
Synonyms : MMP2; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); CLG4, CLG4A, matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase) , matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); 72 kDa type IV collagenase; TBE 1; MMP-2; gelatinase A; 72 kDa gelatinase; collagenase type IV-A; neutrophil gelatinase; matrix metalloproteinase-2; matrix metalloproteinase-II; CLG4; MONA; CLG4A; TBE-1; MMP-II;
Gene ID : 4313
mRNA Refseq : NM_001127891
Protein Refseq : NP_001121363
MIM : 120360
UniProt ID : P08253

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How to regulate the activity of MMP2 in tumor therapy? 10/20/2022

The activity of MMP2 is regulated by inhibitors and antibodies against it to inhibit tumor metastasis and invasion.

How is MMP2 related to metastases that promote disease progression? 05/04/2022

MMP2 interacts with some transfer factors, such as VEGF and EGF, to promote tumor metastasis and invasion.

What are the application prospects of MMP2 inhibitors? 02/20/2022

Inhibitors of MMP2 can be used as potential drug targets for the treatment of tumors, cardiovascular diseases and other related diseases.

Does MMP2 interact with the tumor microenvironment? 09/15/2021

MMP2 interacts with tumor cells, immune cells, blood vessels, etc. in the tumor microenvironment to affect tumor progression and treatment effect.

What is the role of MMP2 in embryonic development? 10/18/2020

It is involved in important processes such as morphogenesis, organ development and intraembryonic lymphatic vessel formation during embryonic development.

What are the effects of MMP2 mutations in human disease? 10/13/2020

Mutations in MMP2 may affect its protein structure and function, leading to the occurrence and progression of related diseases.

Customer Reviews (3)

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06/19/2021

    The solubility and ion exchange ability are very good, which is convenient for subsequent experimental treatment and purification.

    05/13/2021

      Kinetic parameters such as enzymatic reaction rate and binding affinity were tested in the early stage, and it was found that the protein performed well.

      03/16/2021

        MMP2 products undergo strict quality control to ensure the consistency and stability of each batch.

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