| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
lFc |
| Protein Length : |
19-135 a.a. |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Human NKp30, Llama IgG2b Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-H6, Fc,Avitag with a linear range of 0.6-20 ng/mL. Immobilized Human B7-H6, His Tag at 5 μg/mL (100 μL/well) can bind Human NKp30, Llama IgG2b Fc Tag with a linear range of 0.6-78 ng/mL. Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag. |
| Molecular Mass : |
This protein carries a llama IgG2b Fc tag at the C-terminus.The protein has a calculated MW of 40.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Proteinlength : |
Leu19-Gly135 |
| Endotoxin : |
Less than 0.01 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
