Active Recombinant Human SDC1 protein, His-Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: SDC1-1845H
• Product Overview: Biotinylated Recombinant Human SDC1 protein(AAH08765)(23-254 aa), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 23-254 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized BBiotinylated Human Syndecan-1, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Syndecan-1 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: SDC1 syndecan 1 [ Homo sapiens ]
• Official Symbol: SDC1
• Synonyms: SDC1; syndecan 1; SDC; syndecan-1; CD138; SYND1; syndecan; syndecan proteoglycan 1; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor
• Gene ID: 6382
• mRNA Refseq: NM_001006946
• Protein Refseq: NP_001006947
• MIM: 186355
• UniProt ID: P18827