Active Recombinant Human STAT3 protein, His-tagged

• Cat.No.: STAT3-0679H
• Product Overview: Recombinant Human STAT3 protein(Gly127-Arg688), fused with His tag, was expressed in E.coli .

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 127-688 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5 with trehalose as protectant.
• Bio-activity: Loaded Human STAT3, His Tag on NTA Biosensor, can bind STAT3 degrader with an affinity constant of 29.4 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 66.4 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE).
• Proteinlength: Gly127-Arg688
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: STAT3 signal transducer and activator of transcription 3 (acute-phase response factor) [ Homo sapiens ]
• Official Symbol: STAT3
• Synonyms: STAT3; signal transducer and activator of transcription 3 (acute-phase response factor); signal transducer and activator of transcription 3; APRF; DNA-binding protein APRF; acute-phase response factor; HIES; FLJ20882; MGC16063
• Gene ID: 6774
• mRNA Refseq: NM_003150
• Protein Refseq: NP_003141
• MIM: 102582
• UniProt ID: P40763