Active Recombinant Human TGFA Protein (Trp40-Ala89), C-His tagged, Animal-free, Carrier-free

• Cat.No.: TGFA-285H
• Product Overview: Recombinant Human TGFA Protein (Trp40-Ala89) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Transforming Growth Factors alpha (TGF-α) is a 5.68 kDa member of the epidermal Growth Factors with 51 amino acid residues. TGF-α is mainly expressed from brain, skin, epithelial cell (pancreatic endocrine cells, urothelial cells, oligodendrocyte precursor cells, etc.). TGF-α is a regulator of cell proliferation and differentiation via bind to the EGFR and to act synergistically with TGF β. TGF-α also associates with myriad forms of cancer.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 0.2 ng/mL. The specific activity of recombinant human TGF alpha is > 5 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 6.49 kDa.
  The protein migrates as 7 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Trp40-Ala89
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: TGFA transforming growth factor alpha [ Homo sapiens (human) ]
• Official Symbol: TGFA
• Synonyms: TGFA; transforming growth factor alpha; TFGA; protransforming growth factor alpha; TGF-alpha
• Gene ID: www.ncbi.nlm.nih.gov/gene/7039
• mRNA Refseq: NM_003236
• Protein Refseq: NP_003227
• MIM: 190170
• UniProt ID: P01135