Active Recombinant Human TGFA Protein (Trp40-Ala89), C-His tagged, Animal-free, Carrier-free

• Cat.No.: TGFA-285H
• Product Overview: Recombinant Human TGFA Protein (Trp40-Ala89) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Transforming Growth Factors alpha (TGF-α) is a 5.68 kDa member of the epidermal Growth Factors with 51 amino acid residues. TGF-α is mainly expressed from brain, skin, epithelial cell (pancreatic endocrine cells, urothelial cells, oligodendrocyte precursor cells, etc.). TGF-α is a regulator of cell proliferation and differentiation via bind to the EGFR and to act synergistically with TGF β. TGF-α also associates with myriad forms of cancer.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 0.2 ng/mL. The specific activity of recombinant human TGF alpha is > 5 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 6.49 kDa.
  The protein migrates as 7 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Trp40-Ala89
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: TGFA transforming growth factor alpha [ Homo sapiens (human) ]
• Official Symbol: TGFA
• Synonyms: TGFA; transforming growth factor alpha; TFGA; protransforming growth factor alpha; TGF-alpha
• Gene ID: www.ncbi.nlm.nih.gov/gene/7039
• mRNA Refseq: NM_003236
• Protein Refseq: NP_003227
• MIM: 190170
• UniProt ID: P01135

Reviews

05/03/2020

Swift and accurate service, essential for research success.

04/14/2020

Invaluable support for insights, a research cornerstone.

12/21/2018

Trustworthy results, supports research consistency.

Q&As

How does TGFA contribute to cell growth and proliferation?

It stimulates cell proliferation and survival, playing a key role in tissue development.

How do genetic variations in TGFA affect tissue repair?

Genetic mutations in TGFA can lead to impaired wound healing and tissue regeneration.

What is the impact of altered TGFA activity on cancer development?

Altered TGFA activity is linked with cancer progression, as it can stimulate uncontrolled cell growth.

What is the primary role of TGFA in cell signaling?

TGFA, a growth factor, is vital for activating signaling pathways that control cell growth and differentiation.

What potential therapeutic applications arise from targeting TGFA in regenerative medicine and oncology?

Targeting TGFA offers potential for treating wounds and cancer by modulating growth factor signaling.

How does TGFA interact with other growth factors and receptors?

TGFA interacts with epidermal growth factor receptors, influencing various cellular processes.

What role does TGFA play in wound healing?

TGFA is important in wound healing, promoting the regeneration of skin and other tissues.