| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
21-138 aa& 25-116 aa |
| Form : |
Lyophilized from sterile PBS, pH 7.4. |
| Bio-activity : |
1.Label Recombinant Human TSH alpha & beta Heterodimer Protein (His Tag) with biotin. Immobilized Recombinant Human TSH Receptor/TSHR (His Tag) at 2 μg/ml (100 μl/well) can bind biotinylated Recombinant Human TSH alpha & beta Heterodimer Protein, the EC50 is 150-450 ng/mL.
2.Loaded Recombinant Human TSHR Protein (Fc Tag) on proA Biosensor, can bind Recombinant Human TSH alpha & beta Heterodimer Protein (His Tag) with an affinity constant of 70.7 nM as determined in BLI assay. |
| Molecular Mass : |
The recombinant heterodimer of Human TSH alpha & beta comprises 221 (103+118) amino acids and has a calculated molecular mass of 25.15 (11.65+13.50) KDa. The apparent molecular mass of human IL12B & IL23A heterodimer is approximately 22.6 kDa and 17.7 kDa respectively in SDS-PAGE. The molecular weight of this protein is around 26.8 kDa verified by SEC-MALS (Routinely tested). |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS. |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
