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Active Recombinant Mouse Ccl3 Protein (69 aa)

Cat.No. : Ccl3-171C
Product Overview : Recombinant Mouse MIP-1 alpha/CCL3 (rmMIP-1α) produced in HEK 293 cells is a single polypeptide chain containing 69 amino acids. A fully biologically active molecule, rmMIP-1αhas a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
  • Specification
  • Gene Information
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Description : MIP-1 alpha/CCL3, also known as LD78 alpha, is an inflammatory chemokine. MIP-1α belongs to the CCL chemokine family, and shares 68% homology with MIP-1β. The mature form of MIP-1α contains 69 amino acids, exists as dimers in solution, and tends to undergo reversible aggregation. The receptors of MIP-1αin vivo are mainly the G-protein coupled receptors CCR1 and CCR5. Upon stimulation by endogenous and exogenous agents such as Interleukin-1β, Interferon-γ, and lipoteichoic acid from gram-positive bacteria, monocytes are able to secrete significant amounts of MIP-1α. MIP-1α augments the adhesions of T lymphocytes, monocytes, and neutrophils to vascular cell adhesion molecule 1. Additionally, in wounds, MIP-1αchemoattracts macrophages in order to accelerate the tissue repair process.
Source : HEK 293
Species : Mouse
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : The EC50 value of mouse MIP-1α/CCL3 on Ca^2+ mobilization assay in CHO-K1/Gα15/mCCR1 cells (human Gα15 and mouse CCR1 stably expressed in CHO-K1 cells) is less than 100 ng/mL.
Molecular Mass : 7.8 kDa, observed by reducing SDS-PAGE.
Protein Length : 69
AA Sequence : APYGADTPTACCFSYSRKIPRQFIV DYFETSSLCSQPGVIFLTKRNRQIC ADSKETWVQEYITDLELNA
Endotoxin : < 0.2 EU/μg, determined by LAL method.
Purity : > 95% as analyzed by SDS-PAGE and HPLC.
Storage : Lyophilized recombinant Mouse MIP-1α/CCL3 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution Mouse MIP-1α/CCL3 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
Storage Buffer : Lyophilized after extensive dialysis against PBS.
Reconstitution : Reconstituted in ddH2O or PBS at 100 μg/mL.
Gene Name : Ccl3 chemokine (C-C motif) ligand 3 [ Mus musculus ]
Official Symbol : Ccl3
Synonyms : CCL3; chemokine (C-C motif) ligand 3; C-C motif chemokine 3; TY-5; L2G25B; MIP1 (a); SIS-alpha; MIP-1 alpha; MIP-1-alpha; small inducible cytokine A3; small-inducible cytokine A3; heparin-binding chemotaxis protein; macrophage inflammatory protein-1alpha; macrophage inflammatory protein 1-alpha; Mip1a; Scya3; G0S19-1; AI323804; MIP1-(a); LD78alpha; MIP-1alpha; MIP1-alpha;
Gene ID : 20302
mRNA Refseq : NM_011337
Protein Refseq : NP_035467
UniProt ID : P10855

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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