Active Recombinant Mouse Growth Differentiation Factor 9

• Cat.No.: Gdf9-726M
• Product Overview: Recombinant Mouse Gdf9protein was expressed in CHO. Gly307-Arg441.

Specification

• Cat. No.: Gdf9-726M
• Description: Gdf9 is a member ofthe transforming growth factor β (TGF-β) superfamily, and is an oocytesecreted paracrine factor essential for mammalian ovarian folliculogenesis.Mouse Gdf9 is synthesized as a 441 amino acid (aa) prepropeptide thatcontains a 29 aa signal sequence, a 277 aa propeptide, and a 135 aa maturechain). Residues 340-441 constitute a TGF-β like domain. In addition, thereis one potential site of N-linked glycosylation in the mature chain.
• Source: Chinese HamsterOvary cell line, CHO-derived.
• Form: Lyophilized from a0.2 μm filtered solution in HCl with BSA as a carrier protein.
• N-terminalSequence: Gly307
• Molecular Weight: 15.6 kDa
• Activity: Measuredby its ability to induce Smad2 phosphorylation in P19 mouse embryonalcarcinoma cells. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653. 100ng/mL rmGdf9 can effectively induce Smad2 phosphorylation.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 20 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in 4 mM HCl that containing 0.1% bovine serum albumin.
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.
• Tag: Non
• Protein length: Gly307-Arg441

Gene Information

• Gene Name: Gdf9 growth differentiationfactor 9 [ Mus musculus ]
• Official Symbol: Gdf9
• Synonyms: Gdf9; growthdifferentiation factor 9; Gdf-9; growth/differentiation factor 9
• Gene ID: 14566
• mRNA Refseq: NM_008110
• Protein Refseq: NP_032136
• UniProt ID: Q07105
• Chromosome Location: 11 B1.3; 11 29.0 cM
• Pathway: Ovarian InfertilityGenes; PluriNetWork
• Function: cytokine activity;growth factor activity

Reviews

11/14/2021

Expertise in peptide synthesis, accelerates our peptide-related projects.

08/24/2020

Swift protein quantification, improves data reliability in our experiments.

01/31/2019

Outstanding protein-protein interaction studies, boost our research efforts.

Q&As

How does GDF9 influence the proliferation and differentiation of granulosa cells in the ovary?

GDF9 stimulates granulosa cell proliferation and differentiation, essential for healthy ovarian function.

What are the mechanisms by which GDF9 modulates intra-ovarian signaling pathways?

GDF9 interacts with intra-ovarian signaling pathways, coordinating follicle development and ovulation.

How do alterations in GDF9 expression or function affect embryonic development and early pregnancy?

Changes in GDF9 expression impact embryonic development and the success of early pregnancy stages.

What role does GDF9 play in fertility and reproductive outcomes in females?

GDF9 significantly impacts female fertility, affecting egg quality and reproductive potential.

What is the impact of GDF9 mutations on the pathogenesis of ovarian dysfunctions and disorders?

Mutations in GDF9 can lead to ovarian disorders, impacting follicle development and female fertility.

How does GDF9 regulate ovarian follicle development and oocyte maturation?

GDF9 is crucial for ovarian follicle growth and oocyte maturation, influencing female fertility.

How does GDF9 contribute to the regulation of steroidogenesis in ovarian cells?

GDF9 regulates steroid hormone production in ovarian cells, influencing reproductive hormone balance.