Active Recombinant Mouse Il23r, His-tagged
|Product Overview :||A DNA sequence encoding the mouse IL23A (Q9EQ14) (Met1-Ala196 ) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding mouse IL12B (P43432) (Met1-Ser335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse IL23 heterodimer was purified.|
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|Description :||Interleukin-23 subunit alpha, also known as IL-23 subunit alpha, IL-23-A, Interleukin-23 subunit p19, IL-23p19 and IL23A, is a secreted protein which belongs to the IL-6 superfamily. IL-23A associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.Interleukin-12 subunit beta, also known as cytotoxic lymphocyte maturation factor 40 kDa subunit, NK cell stimulatory factor chain 2, NKSF2 and IL12B, is secreted protein which belongs to the type I cytokine receptor family and Type 3 subfamily. IL12B / NKSF2 is a ctokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. IL12B / NKSF2 associates with IL-23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues.|
|Source :||Human Cells|
|Predicted N Terminal :||Val 22 & Met 23|
|Form :||Lyophilized from sterile PBS, pH 7.4.|
|Bio-activity :||Measured by its binding ability in a functional ELISA.1. Measured by its ability to bind recombinant rat IL23R-Fc in a functional ELISA.2. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.14-0.35 μg/ml.3.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/ml (100 μl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.05-0.11 μg/ml.|
|Molecular Mass :||The recombinant mouse IL23 (mouse IL23A&IL12B Heterodimer) comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.3 (21.1+ 37.2) KDa. The apparent molecular mass of the recombinant protein is approximately 47,45 and 26 KDa respectively in SDS-PAGE under reducing conditions.|
|Endotoxin :||< 1.0 EU per μg of the protein as determined by the LAL method|
|Purity :||(33.1+31.5+32.9) % as determined by SDS-PAGE|
|Stability :||Samples are stable for up to twelve months from date of receipt at -70℃|
|Storage :||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution :||A hardcopy of COA with reconstitution instruction is sent along with the products.|
|Gene Name :||Il23r interleukin 23 receptor [ Mus musculus ]|
|Official Symbol :||Il23r|
|Synonyms :||IL23R; interleukin 23 receptor; interleukin-23 receptor; IL-23 receptor; IL-23R;|
|Gene ID :||209590|
|mRNA Refseq :||NM_144548|
|Protein Refseq :||NP_653131|
|Pathway :||Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;|
|Function :||interleukin-23 binding; interleukin-23 receptor activity; receptor activity;|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
IL23R activation promotes Th17 cell differentiation.
IL23R has multiple alternative splicing variants.
IL23R undergoes phosphorylation that affects its stability.
The half-life of IL23R protein in activated T-cells is approximately 48 hours.
MicroRNAs like miR-20a regulate IL23R expression.
IL23R contributes to the release of pro-inflammatory cytokines like IL-17.
IL23R is implicated in autoimmune diseases like Crohn's.
IL23R expression is upregulated during bacterial infection.
IL23R forms heterodimers with IL-12Rβ1.
IL23R activates the JAK-STAT and NF-κB pathways.
Customer Reviews (3)Write a review
High specificity in ELISA. -
Effective in autoimmune models. -
Suitable for receptor binding studies. -
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