Active Recombinant Mouse Il27 Protein

• Cat.No.: Il27-3518M
• Product Overview: Purified recombinant protein of Mouse interleukin 27 (Il27) without tag was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Description: Broad expression in genital fat pad adult (RPKM 5.8), spleen adult (RPKM 2.5) and 18 other tissues
• Bio-activity: The ED50 is 5 - 10 ng/mL, corresponding to a specific activity of 1.0 - 2.0 × 10^5 units/mg, determined by inhibition of IL-2 production induced by mouse IL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.
• Molecular Mass: 49 kDa
• Endotoxin: Less than 0.01 ng/μg protein as determined by the LAL method
• Purity: > 95%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 μg/mL in specified buffer
• Storage Buffer: 20 mM NaH2PO4, pH 6, 0.60 M NaCl

Gene Information

• Gene Name: Il27 interleukin 27 [ Mus musculus (house mouse) ]
• Official Symbol: Il27
• Synonyms: Il27; interleukin 27; Il; p2; IL-; p28; Il30; IL-27; IL27-A; IL-27-A; IL-27p28; interleukin-27 subunit alpha; IL-27 p28 subunit; IL-27 subunit alpha; interleukin 30
• Gene ID: 246779
• mRNA Refseq: NM_145636
• Protein Refseq: NP_663611
• UniProt ID: Q8K3I6