Active Recombinant Mouse IL27 protein, His-Avi-tagged, Biotinylated

• Cat.No.: IL27-0234M
• Product Overview: Biotinylated Recombinant Mouse IL27 protein(Tyr19-Pro228(IL-27B)&Phe29-Ser234(IL-27A)), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His
• Protein Length: 19-228;29-234 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM MOPS, 150 mM NaCl, 0.05% CHAPS, 1mM EDTA, pH7.0. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human IL-27 Ra, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-27 Protein, His,Avitag with a linear range of 0.2-4 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 52.2 kDa. The protein migrates as 55 kDa (IL-27B) & 60 kDa (IL-27A) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Tyr19-Pro228(IL-27B)&Phe29-Ser234(IL-27A)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il27 interleukin 27 [ Mus musculus ]
• Official Symbol: IL27
• Synonyms: IL27; interleukin 27; interleukin-27 subunit alpha; IL27-A; IL-27-A; interleukin 30; IL-27 p28 subunit; IL-27 subunit alpha; p28; Il30; IL-27; IL-27p28
• Gene ID: 246779
• mRNA Refseq: NM_145636
• Protein Refseq: NP_663611