Recombinant Mouse Il27 Protein, His-tagged

• Cat.No.: Il27-2307M
• Product Overview: Recombinant Mouse Il27 Protein (Phe29-Ser234) with a N-His tag was expressed in 293F.

Specification

• Description: Enables interleukin-27 receptor binding activity. Acts upstream of or within positive regulation of defense response to virus by host and regulation of T cell proliferation. Located in extracellular space. Is expressed in decidua; embryo; and extraembryonic component. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; and pulmonary tuberculosis. Orthologous to human IL27 (interleukin 27).
• Source: HEK293F
• Species: Mouse
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.4 kDa
  Accurate Molecular Mass: 28/30 kDa
• Protein length: Phe29-Ser234
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Il27 interleukin 27 [ Mus musculus (house mouse) ]
• Official Symbol: Il27
• Synonyms: Il27; interleukin 27; p28; Il30; IL-27; IL27-A; IL-27-A; IL-27p28; interleukin-27 subunit alpha
  IL-27 p28 subunit
  IL-27 subunit alpha
  interleukin 30
• Gene ID: 246779
• mRNA Refseq: NM_145636
• Protein Refseq: NP_663611
• UniProt ID: Q8K3I6

Reviews

03/30/2022

The purity is very high, no impurities.

08/05/2020

Biological activity is very strong and can be used in various biological experiments.

05/30/2019

Stability is very good, and it will not denaturate at high temperatures.

Q&As

Are there have current difficulties in the study of Il27?

The current challenges in the study of Il27 include potential side effects, preparation purity and other challenges.

Which scientific research fields are deeply studying Il27?

The potential application of Il27 in tumor immunotherapy and autoimmune diseases is being deeply studied in the scientific research field.

Is the production of Il27 related to genetic factors?

The production of Il27 is related to genetic factors, and the expression level and polymorphism of genes may affect its production and function.

Which role of Il27 in inflammatory diseases?

Studies have shown that Il27 may have an anti-inflammatory effect in inflammatory diseases, regulating the degree of inflammatory response.

What the potential applications of recombinant Il27 in cancer therapy?

Current studies have shown that Il27 has potential application value in enhancing anti-tumor immunity and alleviating tumor invasion and metastasis.

What is the effect of Il27 on immune response?

Il27 can enhance the activation and proliferation of specific immune cells and regulate the intensity and direction of immune response.