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Active Recombinant Mouse Il34 protein, His-tagged

Cat.No. : Il34-152M
Product Overview : Recombinant Mouse Il34(Asn21-Pro235) fused with His tag at C-terminal was expressed in NS0.
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  • Gene Information
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Description : Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77 homolog) is a 39 kDa, secreted cytokine that belongs to no known cytokine family. Mouse IL-34 is synthesized as a 235 amino acid (aa) precursor with a 20 aa signal sequence and a 215 aa mature chain. The mature chain contains two potential sites for N-linked glycosylation. There are three isoforms for IL-34. Isoform 1 is the recombinant mouse IL-34 described in this insert. Isoform 2 has an 85 aa substitution for the final 101 aa’s in isoform 1, and isoform 3 lacks Q81 in isoform 1. Mouse IL-34 is 71% identical to human IL-34 on the amino acid level. IL-34 is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and it is most abundant in the spleen. The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). IL-34 stimulates monocyte viability. In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.
Source : Mammalian cells
Species : Mouse
Tag : His
Predicted N Terminal : Asn21
Form : Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 10-40 ng/mL.
Molecular Mass : Predicted Molecular Mass: 25.4 kDa;SDS-PAGE: 35-40 kDa, reducing conditions.
Protein length : Asn21-Pro235
Endotoxin : <0.01 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Gene Name : Il34 interleukin 34 [ Mus musculus ]
Official Symbol : Il34
Synonyms : IL34; interleukin 34; interleukin-34; IL-34; AI593503; 2010004A03Rik;
Gene ID : 76527
mRNA Refseq : NM_001135100
Protein Refseq : NP_001128572
UniProt ID : Q8R1R4
Chromosome Location : 8; 8 E1
Function : cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding; macrophage colony-stimulating factor receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the effect of IL34 on tumor prognosis? 09/19/2022

There are no clear studies showing the effect of IL34 on tumor prognosis.

What are the advantages of IL34 over other treatments? 05/14/2022

Can fight disease by modulating the immune system and may have better efficacy and fewer side effects than other treatments.

What is the mechanism of action of IL34 in the treatment of tumor? 07/09/2020

May inhibit tumor growth and metastasis by enhancing immune cells' ability to attack tumor cells.

How is the clinical trial of IL34 progressing? 04/02/2020

No large-scale clinical trials have been reported, but some early studies have shown a potential therapeutic effect.

How does IL34 affect the immune system? 03/26/2020

Regulate the function of macrophages and dendritic cells and affect the immune response of the immune system.

How to evaluate the efficacy of IL34? 01/31/2019

The evaluation of the efficacy of IL34 usually requires a comprehensive evaluation of clinical symptoms, biochemical indicators, imaging and other indicators.

Customer Reviews (3)

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Reviews
05/10/2021

    IL34 has shown good biocompatibility and safety in related experiments.

    01/19/2020

      When using IL34 for experiments, the experimental conditions and results are highly repeatable.

      10/24/2019

        Quality is stable and reliable, with no significant differences between batches.

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