Active Recombinant Mouse Il34 protein, His-tagged
Cat.No. : | Il34-152M |
Product Overview : | Recombinant Mouse Il34(Asn21-Pro235) fused with His tag at C-terminal was expressed in NS0. |
- Specification
- Gene Information
- Related Products
Description : | Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77 homolog) is a 39 kDa, secreted cytokine that belongs to no known cytokine family. Mouse IL-34 is synthesized as a 235 amino acid (aa) precursor with a 20 aa signal sequence and a 215 aa mature chain. The mature chain contains two potential sites for N-linked glycosylation. There are three isoforms for IL-34. Isoform 1 is the recombinant mouse IL-34 described in this insert. Isoform 2 has an 85 aa substitution for the final 101 aa’s in isoform 1, and isoform 3 lacks Q81 in isoform 1. Mouse IL-34 is 71% identical to human IL-34 on the amino acid level. IL-34 is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and it is most abundant in the spleen. The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). IL-34 stimulates monocyte viability. In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures. |
Source : | Mammalian cells |
Species : | Mouse |
Tag : | His |
Predicted N Terminal : | Asn21 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Bio-activity : | Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 10-40 ng/mL. |
Molecular Mass : | Predicted Molecular Mass: 25.4 kDa;SDS-PAGE: 35-40 kDa, reducing conditions. |
Protein length : | Asn21-Pro235 |
Endotoxin : | <0.01 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Gene Name : | Il34 interleukin 34 [ Mus musculus ] |
Official Symbol : | Il34 |
Synonyms : | IL34; interleukin 34; interleukin-34; IL-34; AI593503; 2010004A03Rik; |
Gene ID : | 76527 |
mRNA Refseq : | NM_001135100 |
Protein Refseq : | NP_001128572 |
UniProt ID : | Q8R1R4 |
Chromosome Location : | 8; 8 E1 |
Function : | cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding; macrophage colony-stimulating factor receptor binding; |
Products Types
◆ Lysates | ||
IL34-2783MCL | Recombinant Mouse IL34 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (6)
Ask a questionThere are no clear studies showing the effect of IL34 on tumor prognosis.
Can fight disease by modulating the immune system and may have better efficacy and fewer side effects than other treatments.
May inhibit tumor growth and metastasis by enhancing immune cells' ability to attack tumor cells.
No large-scale clinical trials have been reported, but some early studies have shown a potential therapeutic effect.
Regulate the function of macrophages and dendritic cells and affect the immune response of the immune system.
The evaluation of the efficacy of IL34 usually requires a comprehensive evaluation of clinical symptoms, biochemical indicators, imaging and other indicators.
Customer Reviews (3)
Write a reviewIL34 has shown good biocompatibility and safety in related experiments.
When using IL34 for experiments, the experimental conditions and results are highly repeatable.
Quality is stable and reliable, with no significant differences between batches.
Ask a Question for All Il34 Products
Required fields are marked with *
My Review for All Il34 Products
Required fields are marked with *
Inquiry Basket