| Cat.No. : |
Il1r1-5651M
Optional Service: Optional requirements on this protein
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| Product Overview : |
RecombinantMouse Il1r1 protein was expressed in Spodopterafrugiperdawith the Fc part of Human IgG1 Pro100-Lys330 (C-terminus) and MouseIl1r1 Phe14-Glu355 (N-terminus). |
| Description : |
Twodistinct types of receptors that bind the pleiotropic cytokines IL-1α andIL-1β have been described. The Il1r1 is an 80 kDa transmembrane protein thatis expressed predominantly by T cells, fibroblasts, and endothelialcells. IL-1receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils,monocytes, large granular leukocytes and endothelial cells. Both receptorsare members of the immunoglobulin superfamily and show approximately 28% sequencesimilarity in their extracellular domains. The two receptor types do not heterodimerizein a receptor complex. |
| Source : |
Spodopterafrugiperda, Sf 21 (baculovirus)-derived. |
| Form : |
Lyophilizedfrom a 0.2 um filtered solution in PBS. |
| N-terminalSequence : |
Phe14 |
| MolecularWeight : |
64.7 kDa (monomer) |
| Activity : |
Measuredby its ability to inhibit IL-1α-dependent proliferation in D10.G4.1 mousehelper T cells. Approximately 2-8 μg/mL of IL-1 RII will inhibit 50% of thebiological response due to 50 pg/mL of recombinant mouse IL-1α. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
70-80kDa, reducing conditions. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
500 μg/mLin sterile PBS |
| Storage : |
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution. |
| PDBrendering based on 1g0y. : |
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