Il1r1-5651M
Optional Service: Optional requirements on this protein
Product Overview :
RecombinantMouse Il1r1 protein was expressed in Spodopterafrugiperdawith the Fc part of Human IgG1 Pro100-Lys330 (C-terminus) and MouseIl1r1 Phe14-Glu355 (N-terminus).
Description :
Twodistinct types of receptors that bind the pleiotropic cytokines IL-1α andIL-1β have been described. The Il1r1 is an 80 kDa transmembrane protein thatis expressed predominantly by T cells, fibroblasts, and endothelialcells. IL-1receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils,monocytes, large granular leukocytes and endothelial cells. Both receptorsare members of the immunoglobulin superfamily and show approximately 28% sequencesimilarity in their extracellular domains. The two receptor types do not heterodimerizein a receptor complex.
Source :
Spodopterafrugiperda, Sf 21 (baculovirus)-derived.
Form :
Lyophilizedfrom a 0.2 um filtered solution in PBS.
N-terminalSequence :
Phe14
MolecularWeight :
64.7 kDa (monomer)
Activity :
Measuredby its ability to inhibit IL-1α-dependent proliferation in D10.G4.1 mousehelper T cells. Approximately 2-8 μg/mL of IL-1 RII will inhibit 50% of thebiological response due to 50 pg/mL of recombinant mouse IL-1α.
Endotoxin Level :
<1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE :
70-80kDa, reducing conditions.
Purity :
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution :
500 μg/mLin sterile PBS
Storage :
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.