Active Recombinant Rat Mitogen Activated Protein Kinase Kinase Kinase 1

• Cat.No.: Map3k1-665R
• Product Overview: Recombinant Rat Mitogen Activated Protein Kinase Kinase Kinase 1 is produced inEscherichia coli.MW= 42 kDa.

Specification

• Cat. No.: Map3k1-665R
• Description: MAP kinase is a serine/threonine kinase that is activated by MEK through a phosphorylation cascade. The cascade starts with extracellular signals transmitted by growth factor-regulated receptor tyrosine kinases or G-protein coupled receptors. Dually phosphorylated (Thr and Tyr) MAP kinase translocates from the cytoplasm to the nucleus, where it phosphorylates protein transcription factors, transducing extracellular signals into genomic responses, proliferation or differentiation.
• Source: E. coli.
• Purity: ≥95% (SDS-PAGE).
• Unit Definition: One unit of activated MAP kinase will transfer 1 nmole of phosphate from ATP to myelin basic protein per min at 30 °C.
• Physical Form: Solution in 20 mM Tris, pH 7.5, 150 mM NaCl, 1 mM EGTA, 10% glycerol, 1 mM DTT, and 0.03% Brij.
• Form: Buffered aqueous glycerol solution.
• Activity: ≥500 units/mg.
• Legal Information: Brij is a registered trademark of Croda International PLC.
• Storage: −70°C. Shipped in dry ice.
• Tag: Non

Gene Information

• Gene Name: Map3k1 mitogen activated protein kinase kinase kinase 1 [ Rattus norvegicus ]
• Synonyms:
  Map3k1; mitogen activated protein kinase kinase kinase 1; Mekk1; mitogen activated protein kinase kinase kinase 1; MEK kinase 1; mitogen-activated protein kinase kinase kinase 1; MAPK/ERK kinase kinase 1; EC 2.7.11.25
• Gene ID: 116667
• mRNA Refseq: NM_053887
• Protein Refseq: NP_446339
• UniProt ID: Q62925
• Chromosome Location: 2q14
• Pathway: GnRH signaling pathway; MAPK signaling pathway; Neurotrophin signaling pathway; RIG-I-like receptor signaling pathway; Ubiquitin mediated proteolysis
• Function: ATP binding; JUN kinase binding; JUN kinase kinase kinase activity; MAP kinase kinase kinase activity; identical protein binding; mitogen-activated protein kinase kinase binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity; ubiquitin-protein ligase activity; zinc ion binding

Reviews

12/20/2020

The bands of proteins in Western Blotting experiments were very clear.

11/10/2020

The MAP3K1 protein is highly recommended for its exceptional performance in ELISA assays.

09/30/2017

Researchers can rely on the MAP3K1 protein to deliver precise results, enhancing the credibility and efficiency of their experiments.

Q&As

How is MAP3K1 protein expression measured in clinical settings?

MAP3K1 protein expression can be measured using immunohistochemistry (IHC) or Western blotting in clinical settings.

Are there any diseases associated with MAP3K1 protein mutations?

Yes, mutations in the MAP3K1 gene have been linked to several diseases, including breast cancer, lung cancer, and colon cancer.

What are the implications of MAP3K1 protein in drug resistance?

MAP3K1 protein has been implicated in the development of drug resistance in certain cancer types, highlighting its importance in personalized medicine and treatment response.

What are the potential diagnostic applications of MAP3K1 protein analysis?

MAP3K1 protein analysis can be used for diagnostic purposes to identify specific molecular subtypes of cancer and to predict disease prognosis.

How is MAP3K1 protein targeted in precision medicine approaches?

Precision medicine approaches may involve targeting MAP3K1 protein through specific inhibitors or monoclonal antibodies to inhibit its oncogenic activity.