Active Recombinant Rat TNF Protein

Cat.No. : TNF-251R
Product Overview : Recombinant Rat TNF Protein without tag was expressed in E. coli.
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Description : Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.
Source : E. coli
Species : Rat
Bio-activity : Cytolysis of mouse L929 cells in the presence of Actinomycin D, ≤50 pg/mL
Molecular Mass : Monomer, 17.3 kDa (157 aa)
AA Sequence : MLRSSSQNSSDKPVAHVVANHQAEE QLEWLSQRANALLANGMDLKDNQLV VPADGLYLIYSQVLFKGQGCPDYVL LTHTVSRFATSYQEKVSLLSAIKSP CPKDTPEGAELKPWYEPMYLGGVSQ LEKGDLLSAEVNLPKYLDITESGQV YFGVIAL
Endotoxin : ≤1 EUs/μg, Kinetic LAL
Purity : ≥95%, Reducing and Non-Reducing SDS PAGE
Stability : 12 months from date of receipt when stored at -20 to -80 centigrade as supplied.
1 month when stored at 4 centigrade after reconstituting as directed.
3 months when stored at -20 to -80 centigrade after reconstituting as directed.
Storage : Storage Prior to Reconstitution: -20 centigrade
Storage Buffer : Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Reconstitution : Sterile water at 0.1 mg/mL
Shipping : Room temperature
Instructions : Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 centigrade and avoid repeat freeze thaws.
Gene Name : Tnf tumor necrosis factor [ Rattus norvegicus (Norway rat) ]
Official Symbol : TNF
Synonyms : TNF; tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; Tnfa; RATTNF; TNF-alpha; MGC124630;
Gene ID : 24835
mRNA Refseq : NM_012675
Protein Refseq : NP_036807
UniProt ID : P16599

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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