Recombinant Human TNF, His-tagged TNF-30945TH

Recombinant Human TNF, His-tagged

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Cat.No. : TNF-30945TH Optional Service: Optional requirements on this protein
Product Overview : Recombinant full length soluble Human TNF alpha with His tag produced in the endosperm tissue of barley grain ; 174 amino acids with tag, Predicted MWt 19.6 kDa.
Description : This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Conjugation : HIS
Biological activity : Bioactivity:The ED50 is determined by the Cytotoxicity Assay for Human TNF alpha for dose dependent cytolytic effects of serial dilutions of hTNF alpha on the fibrosarcoma L-929 cells. The ED50 for TNF-30945TH was determined to be 40 ng/ml corresponding t
Form : Lyophilised:Reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 μg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).
Purity : >95% by SDS-PAGE
Storage buffer : Preservative: NoneConstituents: PBS, pH 7.2
Storage : The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.
Sequence Similarities : Belongs to the tumor necrosis factor family.
Gene Name : TNF tumor necrosis factor [ Homo sapiens ]
Official Symbol : TNF
Synonyms : TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2;
Gene ID : 7124
mRNA Refseq : NM_000594
Protein Refseq : NP_000585
MIM : 191160
Uniprot ID : P01375
Chromosome Location : 6p21.3
Pathway : Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem;
Function : cytokine activity; identical protein binding; protease binding; protein binding; transcription regulatory region DNA binding;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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