Recombinant Human MLF1 protein, His-tagged

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Recombinant Human MLF1 protein, His-tagged

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Cat.No. : MLF1-3438H
Product Overview : Recombinant Human MLF1(1-268aa) fused with His tag at N-terminal was expressed in E. coli.
Description : MLF1 (Myeloid leukemia factor 1) belongs to MLF family, and is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. This protein interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 normally functions in multi-potent progenitor cells, and its dysregulation may be somewhat responsible for leukemogenesis.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 5mM DTT, 200mM NaCl
Molecular Mass : 32.8kDa (288aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
AA Sequence : MGSSHHHHHH SSGLVPRGSHSFQTMDQMVS NMRNYMQKLE RNFGQLSVDP NGHSFCSSSV MTYSKIGDEP PKVFQASTQT RRAPGGIKET RKAMRDSDSG LEKMAIGHHI MFRMLNSSFE DDPFFSESIL AHRENMRQMI RSFSEPFGRD LLSISDGRGR AHNRRGHNDG EDSLTHTDVSHDRAHVIKKS KNKKTGDEEV NQEFINMNES DAHAFDEEWQ SEVLKYKPGR HNLGNTRMRS VGHENPGSRE LKRREKPQQS PAIEHGRRSNVLGDKLHIKG SSVKSNKK
Purity : >85% by SDS - PAGE
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.5 mg/ml (determined by Bradford assay)
Gene Name : MLF1 myeloid leukemia factor 1 [ Homo sapiens ]
Official Symbol : MLF1
Synonyms : MLF1; myeloid leukemia factor 1; myeloid leukemia factor 1 variant 1; myeloid leukemia factor 1 variant 2; myeloid leukemia factor 1 variant 3; myelodysplasia-myeloid leukemia factor 1;
Gene ID : 4291
mRNA Refseq : NM_022443
Protein Refseq : NP_071888
MIM : 601402
UniProt ID : P58340
Chromosome Location : 3q25
Pathway : Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;
Function : DNA binding; DNA binding; protein binding; protein domain specific binding;

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