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Recombinant Human ENOPH1 protein, His-tagged

Cat.No. : ENOPH1-3457H
Product Overview : Recombinant Human ENOPH1(1-261aa) fused with His tag at N-terminal was expressed in E. coli.
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  • Gene Information
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Description : Enolase-phosphatase E1, also known as ENOPH1, is a member of the MasA family of the HAD (halo-acid dehalogenase)-like hydrolase superfamily. ENOPH1 is a bifunctional enzyme, exhibiting both phosphatase and atypical enolase activities. ENOPH1 plays an important role in the ubiquitous methionine salvage pathway, a biochemical pathway found in all organisms that regulate methionine levels in the cell.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
Molecular Mass : 31 kDa (281aa), confirmed by MALDI-TOF
AA Sequence : MGSSHHHHHH SSGLVPRGSHGAVPIPAASG NGVDDLQQMI QAVVDNVCWQ MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY IYSSGSVEAQKLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLITMVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE ECQQDVSLLR KQAEEDAHLDSFSELYLPSS T
Purity : >90% by SDS - PAGE
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Bradford assay)
Gene Name : ENOPH1 enolase-phosphatase 1 [ Homo sapiens ]
Official Symbol : ENOPH1
Synonyms : ENOPH1; enolase-phosphatase 1; enolase-phosphatase E1; acireductone synthase; E1; Enolase phosphatase E1; MASA; MASA homolog; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase; MST145; FLJ12594; DKFZp586M0524;
Gene ID : 58478
mRNA Refseq : NM_021204
Protein Refseq : NP_067027
MIM :
UniProt ID : Q9UHY7
Chromosome Location : 4q21.3
Pathway : Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Metabolism of polyamines, organism-specific biosystem; Methionine salvage pathway, organism-specific biosystem; Methionine salvage pathway, organism-specific biosystem;
Function : 2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity; 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity; acireductone synthase activity; acireductone synthase activity; hydrolase activity; magnesium ion binding; phosphoglycolate phosphatase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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