Recombinant Human MAP3K14, GST-tagged

• Cat.No.: MAP3K14-556H
• Product Overview: Recombinant Human MAP3K14 (a.a. 381-end) with N-terminal GST tag expressed in aBaculovirus infected Sf9 cellexpression system. MW = 96 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 381-end a.a.
• Description: Mitogen-activated protein kinase kinase kinase 14 is an enzyme that in humans is encoded by the MAP3K14 gene. This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Purity: >90%.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
• Specific Activity: 20 nM NIK yields 20% conversion of 1 uM [32P]ATP using MBP as substrate. Reaction condition: 10 mM HEPES, pH 7.4, 5 mM MgCl2, 10 μM MnCl2, 1 mM DTT, 3 μM Na-orthovanadate, 1 μM ATP, and MBP as substrate at room temperature for 2 hrs.
• Stability: >6 months at -80°C.

Gene Information

• Gene Name: MAP3K14 mitogen-activated protein kinase kinase kinase 14 [ Homo sapiens ]
• Synonyms: mitogen-activated protein kinase kinase kinase 14; HS; NIK; HSNIK; FTDCR1B; MAP3K14; NF-kappa-beta-inducing kinase; serine/threonine protein-kinase; serine/threonine-protein kinase NIK; EC 2.7.11.25; Serine/threonine-protein kinase NIK
• Gene ID: 9020
• mRNA Refseq: NM_003954.2
• Protein Refseq: NP_003945.2
• MIM: 604655
• UniProt ID: Q99558
• Chromosome Location: 17q21
• Pathway: Apoptosis; Epithelial cell signaling in Helicobacter pylori infection; Intestinal immune network for IgA production; MAPK signaling pathway; T cell receptor signaling pathway
• Function: MAP kinase kinase kinase activity; ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity