Recombinant Mouse Fibroblast Growth Factor 2


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Recombinant Mouse Fibroblast Growth Factor 2

Fgf2 Related Products

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Cat. No. : Fgf2-680M
Product Overview : Recombinant Mouse Fgf2is Expressed in E. coli.
Description : Fgf2 is one of atleast 22 mitogenic proteins of the FGF family, which show 35-60% amino acidconservation. Storage pools within the cell or on cell surface HeparanSulfate Proteoglycans (HSPG) are likely. The predicted 17 kDa Fgf2 isoformcan be located in both the cytoplasm and the nucleus and is presumed to bethe form secreted. Transcription from alternate start sites produces 21-24kDa forms found only in the nucleus. High and low molecular weight Human Fgf2targets the expression of different genes when expressed in NIH-3T3 cells.
Source : E. coli
Species : Mouse
Form : Lyophilized fromsterile filtered PBS pH 7.4.
Molecular Weight : 17 kDa
Purity : > 98% by HPLC andSDS-PAGE
Endotoxin Level : < 0.1 EU/µg
Biological Activity : ED50,determined by the dose-dependent stimulation of Thymidine uptake by BaF3cells expressing FGF receptors is <0.1ng/mL.
Specific Activity : 1x107Units/mg
Reconstitution : Centrifuge vialprior to opening. Add steriledistilled water or aqueous buffer containing 0.1% BSA to a concentration of0.1-1.0 mg/mL. Please note that the addition of any carrier protein into thisproduct may produce unwanted endotoxin. This depends upon the particularapplication employed. Further dilutions should be made in appropriatebuffered solutions.
Storage : Stable at 2-8°C, butshould be kept desiccated -20°C. Upon reconstitution, stable for up to 1 weekat 2-8°C. For maximal stability, store in working aliquots below -20°C. Avoidrepeated freeze/thaw cycles.
OfficialSymbol : Fgf2
PDBrendering based on 1bas. :

Gene Name : Fgf2 fibroblast growth factor 2[ Mus musculus ]
Synonyms : Fgf2; fibroblastgrowth factor 2; Fgfb; bFGF; Fgf-2; heparin-binding growth factor 2;HBGF-2; OTTMUSP00000046776; OTTMUSP00000047630; basic fibroblast growthfactor
Gene ID : 14173
mRNA Refseq : NM_008006
Protein Refseq : NP_032032
UniProt ID : P15655
Chromosome Location : 3 19.3 cM
Pathway : Downstream signalingof activated FGFR; ESC Pluripotency Pathways; Endochondral Ossification; FGFRligand binding and activation; FRS2-mediated cascade; IRS-mediated signalling;IRS-related events; Insulin receptor signalling cascade; MAPK signalingpathway; Melanoma; MicroRNAs in cardiomyocyte hypertrophy; PI-3K cascade; Pathwaysin cancer; Phospholipase C-mediated cascade
Function : chemoattractantactivity; cytokine activity; fibroblast growth factor binding; fibroblastgrowth factor receptor binding; growth factor activity; heparin binding; ligand-dependentnuclear receptor transcription coactivator activity; protein binding; voltage-gatedcalcium channel activity

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