||Recombinant humanFLT3 is Expressed in HEK 293 cells.
||FLT3 Ligand is expressed from human 293cells as a monomeric glycoprotein with an apparent MW 24 to 30 kDa. Thiscytokine is produced in a serum-free, chemically defined media. The molecularmass is a narrower range than reported when this cytokine is expressed ininsect Sf21 cells (17 to 30 kDa), emphasizing the difference in glycosylationin different expression systems.
||HEK 293 cells
||24 to 30 kDamonomer, glycosylated
||> 95% The proteinwas resolved by SDS polyacrylamide gel electrophoresis and the gel wasstained with Coomassie blue.
||Endotoxin Free <1EU/ug
||0.2 to 0.8 ng/mLED50
||The specificactivity was determined by the dose-dependent stimulation of theproliferation of the human acute myeloid leukemia cell line OCI-AML5.