Recombinant Human Insulin-Like Growth Factor 2 (somatomedin A)

• Cat.No.: IGF2-692H
• Product Overview: Recombinant humanIGF2 was expressed in E. coli.

Specification

• Cat. No.: IGF2-692H
• Description: Human [Leu27]IGF-II is an analog of human IGF-II which has Leu in place of Tyr at position27. Of the IGF-II amino acid sequence. Compared to human IGF-II, human [Leu27]IGF-II has a 10-20 fold reduced affinity for the Type 1 IGF receptor.
• Source: E. coli
• Species: Human
• Purity: Greater than 95% (byHPLC and N-terminal sequence analysis).
• Molecular Weight: 7.420 kDa-confirmedby Mass Spectometry.
• Endotoxin Level: Less than 0.1 EU/ug
• Biological Activity: Type 1 IGF receptorbinding assay: ED50>175 ng/ml. Type 2 IGF receptor binding assay:ED50<371 ng/ml. IGF binding protein assay: ED50<10 ng/ml.
• State and appearance: Lyophilized whitepowder. Dried from 0.1 M acetic acid and stored under dry nitrogen at aslight vacuum (-25 kPa).
• N-terminal sequenceanalysis: 5 residues Greaterthan 95% single sequence.
• Reconstitution: Refer to Protocol 1000:Handling of GroPep IGF-I, IGF-II and IGF Analogs.
• Storage: At least 2 years at2-4°C (lyophilized).
• OfficialSymbol: IGF2

Gene Information

• Gene Name: IGF2 insulin-like growth factor2 (somatomedin A) [ Homo sapiens ]
• Synonyms: IGF2; insulin-likegrowth factor 2 (somatomedin A); IGF-II; PP9974; C11orf43; FLJ22066; FLJ44734;insulin-like growth factor II;somatomedin-A; OTTHUMP00000011012; OTTHUMP00000011013; OTTHUMP00000011015;OTTHUMP00000011018; OTTHUMP00000011157; OTTHUMP00000011158;OTTHUMP00000220677; insulin-like growth factor type 2
• Gene ID: 3481
• mRNA Refseq: NM_000612
• Protein Refseq: NP_000603
• MIM: 147470
• UniProt ID: P01344
• Chromosome Location: 11p15
• Pathway: Apoptosis;Diabetes pathways; Endochondral Ossification; Hedgehog Signaling Pathway;Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-likeGrowth Factor Binding Proteins (IGFBPs)
• Function: growthfactor activity; hormone activity; insulin receptor binding; insulin-likegrowth factor receptor binding; protein binding; protein serine/threoninekinase activator activity; receptor activator activity