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Mouse Anti-Human Carbonic Anhydrase III, Muscle Specific Monoclonal Antibody

Cat.No. : CAB11487MH
Product Overview : Mouse monoclonal antibody to human carbonic anhydrase III, muscle specific.
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  • Gene Information
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Antigen Description : Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site.Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to the alpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
Specificity : Human Carbonic Anhydrase III / CA3. No cross-reactivity in ELISA with Human Carbonic Anhydrase II / CA2, Human Carbonic Anhydrase VA / CA5a, Human Carbonic Anhydrase VIII / CA8, Human Carbonic Anhydrase IX / CA9, Human Carbonic Anhydrase XIII / CA13, Human Carbonic Anhydrase XII / CA12, E.coli cell lysate.
Immunogen : Recombinant Human Carbonic Anhydrase III / CA3 Protein
Host : Mouse
Isotype : Mouse IgG1
Species : Human
Clone : 6H9C7
Purification : Protein A affinity chromatography
Applications : Western Blot; ELISA
Dilution : Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect CA3 in WB. Using a DAB detection system, the detection limit for CA3 is approximately 2 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect CA3. The detection limit for CA3 is approximately 0.16 ng/well.
Preparation : This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human Carbonic Anhydrase III / CA3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format : 0.2 μm filtered solution in PBS with 5% trehalose
Storage : This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name : CA3 carbonic anhydrase III, muscle specific [ Homo sapiens ]
Official Symbol : CA3
Synonyms : CA3; carbonic anhydrase III, muscle specific; carbonic anhydrase 3; CAIII; Car3; CA-III; carbonate dehydratase III; FLJ36434;
Gene ID : 761
mRNA Refseq : NM_005181
Protein Refseq : NP_005172
MIM : 114750
UniProt ID : P07451
Chromosome Location : 8q21.2
Function : carbonate dehydratase activity; lyase activity; metal ion binding; nickel cation binding; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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In which tissues is CA3 expression most prominently observed? 10/22/2022

CA3 is primarily found in skeletal muscles.

What post-translational modifications are characteristic of CA3? 06/23/2022

Post-translational modifications, like phosphorylation or glycosylation, can be identified using mass spectrometry.

Are there known inhibitors that specifically target CA3? 09/19/2021

Specific inhibitors for CA3 are yet to be fully elucidated and would be determined using enzymatic assays.

How does CA3 participate in metabolic processes within the cell? 02/04/2021

CA3 plays a role in CO2 and bicarbonate equilibrium, crucial for metabolic pathways like glycolysis and the citric acid cycle.

How does CA3 enzymatic activity differ from other carbonic anhydrase isoforms? 10/26/2019

While CA3 has a similar mechanism, its activity and affinity might differ from other isoforms, requiring comparative studies.

Are there any pathological conditions directly linked to CA3 dysregulation? 06/08/2017

CA3 dysregulation might be linked to muscle diseases or metabolic disorders, requiring further research.

What is the primary physiological role of CA3 in cells? 05/26/2017

CA3 catalyzes the reversible hydration of carbon dioxide, aiding in pH regulation and CO2 transport.

Customer Reviews (3)

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Reviews
09/23/2022

    Outstanding protein sequencing capabilities, unrivaled accuracy.

    05/23/2022

      Precise purification techniques, yield exceptional samples.

      05/25/2021

        Efficient sample handling, optimizes our laboratory workflow.

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