Recombinant Human Neurotrophin 3 NTF3-3621H

Recombinant Human Neurotrophin 3

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Recombinant Human Neurotrophin 3

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Cat. No. : NTF3-3621H
Product Overview : Recombinant Human proNT-3 produced in E.coli is a single, non-glycosylated, polypeptide chain containing 239 amino acids and having a molecular mass of 27.4 kDa.
Description : The precursor form of neurotrophin-3 (proNT-3) functions via p75NTR and sortilin and induces neuronal apoptosis. On the contrary, mature NT-3 interacts with Trk receptors and selectively promotes the survival, growth and differentiation of neurons. As axon retrograde transport is crucial for neurotrophin signaling, proNT-3 also plays an important role in the mechanism of retrograde signaling.
Biological Activity : As measured by its binding ability in a functional ELISA, immobilized rHuIL-1F10 at 1 μg/ml (100 μl/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15~5 μg/ml.
Purity : Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence : Thesequenc eofthefirs tfiveN-ter minalamino acidswasde terminedan dwasfoundt obeAsn-Asn -Met-Asp-G ln.
Endotoxin : Less than 0.1ng/μg (1.0EU/μg) of rHuproNT-3 as determined by LAL test.
Form : The protein was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 8.0, 300mM NaCl buffer.
Reconstitution : It is recommended to reconstitute the lyophilized rHuproNT-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage : Lyophilized rHuproNT-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuproNT-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Figure :

Gene Name : NTF3 neurotrophin 3 [ Homo sapiens ]
Official Symbol : NTF3
Synonyms : NTF3; NT3; HDNF; NGF2; NGF-2; MGC129711; NT-3; OTTHUMP00000239132; OTTHUMP00000239133; neurotrophic factor; nerve growth factor 2; neurotrophin 3; Nerve growth factor 2; Neurotrophic factor; neurotrophin-3
Gene ID : 4908
mRNA Refseq : NM_002527
Protein Refseq : NP_002518
MIM : 162660
UniProt ID : P20783
Chromosome Location : 12p13
Function : growth factor activity; nerve growth factor binding; neurotrophin receptor binding; protein binding; receptor binding

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