Recombinant Human Epidermal Growth Factor

Cat. No.: EGF-556H
Product Overview: A neurotrophic factor that promotes the survival of various neuronal cell types and may play an important role in the injury response in the nervous system. Recombinant Human Epidermal Growth Factor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6,222 Daltons.
Description: Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth,proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds.
Species: Human
Source: E.coli
Form: Lyophilized with no additives.
Purity: Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity: Recombinant Human Epidermal Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 20.25pg/ml.
Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Amino acid sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Gene Name: EGF epidermal growth factor [ Homo sapiens ]
Official Symbol: EGF
Synonyms: EGF; epidermal growth factor (beta-urogastrone); URG; HOMG4; epidermal growth factor; beta-urogastrone; Pro-epidermal growth factor; Epidermal growth factor; Urogastrone; OTTHUMP00000162922; OTTHUMP00000219721; OTTHUMP00000219722
Gene ID: 1950
mRNA Refseq: NM_001963
Protein Refseq: NP_001954
MIM: 131530
UniProt ID: P01133
Chromosome Location: 4q25
Pathway: Bladder cancer; Cytokine-cytokine receptor interaction; Endocytosis; Endometrial cancer; ErbB signaling pathway; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Regulation of actin cytoskeleton; Hemostasis; Signaling by EGFR
Function: calcium ion binding; epidermal growth factor receptor binding; growth factor activity; protein binding; transmembrane receptor protein tyrosine kinase activator activity

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