Recombinant Human Epidermal Growth Factor

Cat. No.: EGF-632H
Product Overview: Epidermal Growth Factor Human Recombinant produced in Pichia Pastoris is a single, glycosylated, polypeptide chain containing 51 amino acids and having a molecular mass of 6KDa. The EGF is purified by proprietary chromatographic techniques.
Description: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
Source: Human, Pichia
Host: Pichia Pastoris.
Form: Each lyophilized mg contains contains 0.15M NaCl, 0.025M sodium bicarbonate, pH-7.5.
Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Bio-activity: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 10,000,000 Units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility: It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu, which agrees with the sequence of native EGF human. N-terminal methionine has been completely removed enzymatically.
Protein content: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.
Storage: Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: EGF epidermal growth factor [ Homo sapiens ]
Official Symbol: EGF
Synonyms: EGF; URG; HOMG4; pro-epidermal growth factor; beta-urogastrone; OTTHUMP00000162922; OTTHUMP00000219721; OTTHUMP00000219722
Gene ID: 1950
mRNA Refseq: NM_001963
Protein Refseq: NP_001954
MIM: 131530
UniProt ID: P01133
Chromosome Location: 4q25
Pathway: Arf6 signaling events; Bladder cancer; Ceramide signaling pathway; Cytokine-cytokine receptor interaction; Delta-Notch Signaling Pathway; EGF receptor (ErbB1) signaling pathway; EGFR downregulation; EGFR interacts with phospholipase C-gamma; EGFR-dependent Endothelin signaling events; EGFR1 Signaling Pathway; Endocytosis; Endometrial cancer; ErbB receptor signaling network; ErbB signaling pathway; ErbB1 downstream signaling; Focal Adhesion; Formation of Platelet plug; Gab1 signalosome; Gap junction; Glioma; Grb2 events in EGFR signaling; Hemostasis; Hepatitis C; Id Signaling Pathway; Internalization of ErbB1; MAPK signaling pathway; Melanoma; MicroRNAs in cardiomyocyte hypertrophy; on-small cell lung cancer; Pancreatic cancer; Platelet Activation; Platelet degranulation; Prostate cancer; Regulation of Actin Cytoskeleton; Regulation of Telomerase; Regulation of actin cytoskeleton; Signaling events mediated by PTP1B; Stabilization and expansion of the E-cadherin adherens junction; TGF Beta Signaling Pathway
Function: calcium ion binding; epidermal growth factor receptor binding; growth factor activity; protein binding; transmembrane receptor protein tyrosine kinase activator activity

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