Description : |
Cytokine; may be involved in immune system function or to cell death and cell survival. |
Source : |
E.coli |
Species : |
Rat |
Form : |
Lyophilized from a 0.2μm filtered concentrated solution in 2 × PBS, pH 6.5. |
Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg. |
Molecular Mass : |
Approximately 30.0 kDa, a disulfide-linked homodimer of two 133 amino acid polypeptide chains. |
Protein length : |
133 |
AA Sequence : |
AVLIPQSSVCPNAEANNFLQNVKVN LKVINSLSSKASSRRPSDYLNRSTS PWTLSRNEDPDRYPSVIWEAQCRHQ RCVNAEGKLDHHMNSVLIQQEILVL KREPEKCPFTFRVEKMLVGVGCTCV SSIVRHAS |
Endotoxin : |
Less than 0.1 EU/µg of rRtIL-17A as determined by LAL method. |
Purity : |
>95% by SDS-PAGE and HPLC analysis. |
Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |