Active Recombinant Human UDP Glucuronosyltransferase 1 Family, Polypeptide A1

• Cat.No.: UGT1A1-1118H
• Product Overview: Human UGT1A1 (NP_000454, 1 a.a. ~ 154 a.a.) partial recombinant protein expressed in Escheri- chia coli.

Specification

• Cat. No.: UGT1A1-1118H
• Description: UGT-1A is a uridine diphosphate glucuronyltransferase (UDP-glucuronosyltransferase, UDPGT), an enzyme of the glucuronidation pathway that transforms small li- pophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites.
• Sequence: MATKAVCVLKGDGPVQG IINFEQKESNGPVKVWGS IKGLTEGLHGFHVHEFGD NTAGCTSGPHFNPLSRKH GGPKDEERHVGDLGNVTA DKDGVADVSIEDSVISLSGD HCIIGRTLVVHEKADDLGKGGN EESTKTGNAGSRLACGVIGIAQ
• Theoretical MW (kDa): 15.9
• Form: Liquid
• Preparation Method: Escherichia coli expression system
• Purity: μ 95% by SDS-PAGE
• Activity: Specific activity is > 90 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25°C in a 1.5 ml reaction volume.
• Application: SDS-PAGE; Functional Study
• Storage Buffer: In 20 mM Tris, pH 7.5 (10% glycerol)
• Storage: Store at 4°C for 1~2 weeks. For long term storage store at -20°C or -80°C.Aliquot to avoid repeated freezing and thawing.
• Unitprot ID: Q5DT03
• Pathways: Ascorbate and aldarate metabolism; Biological oxidants; Drug metabolish-cytochrome P450; Estrogen metabolish; Irinotecan pathway; Metabolish of lipids and lipoproteins; Retinol metabolish; Starch and sucrose metabolish; Steroid hormone biosynthesis; metabolish pathway
• Functions: enzyme binding; enzyme inhibitor activity; qlucuronosyltransfersase activity; protein heterodimerization activity; retinoic acid binding; steroid binding

Gene Information

• Gene Name: UGT1A1 UDP glucuronosyltransferase 1 family, polypeptide A1 [ Homo sapiens ]
• Official Symbol: UGT1A1
• Synonyms: UGT1A1; GNT1; UGT1; UDPGT; UGT1A; HUG-BR1; BILIQTL1; UDPGT 1-1; HUG-BR1; UDP glycosyltransferase 1 family, polypeptide A1; UGT1-01; UGT-1A; UGT1*1; UGT1.1; UDP-glucuronosyltransfersae 1-A1; UDPGT 1-1; EC 2.4.1.17; BILIQTL1; Hug-br1; UDP glucuronosyltransferase 1 family, polypeptide A1;Bilirubin-specific UDPGT isozyme 1; UDPGT; UDP-glucuronosyltransfersae 1-A; OTTHUMP000000651199
• Gene ID: 54658
• mRNA Refseq: NM_000463
• Protein Refseq: NP_000454
• MIM: 191740
• Chromosome Location: 2q37