||GM-CSF is a highly purified protein with a molecular weight of approximately 14kDa. It contains 123 amino acids residues. The protein is produced by recombinant DNA technology using a genetically engineered E. coli strain containing the human GM-CSF gene.
||GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
||>95% as determined by SDS-PAGE and SEC-HPLC.
||Lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH=7.4+/-0.1.
||The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.1 ng/ml, corresponding to a Specific Activity of 1.0 x 107IU/ mg.
||Less than 0.03ng/µg (0.03IEU/µg) determined by LAL test..
||It is recommended to reconstitute the lyophilized rHuGM-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions
||Lyophilized rHuGM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution rHuGM-CSF should be stored at 4oC between 2-7 days and for future use below -18oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.