Recombinant Human Hypoxia Inducible Factor 1, Alpha Subunit, His-tagged

• Cat.No.: HIF1A-5020H
• Product Overview: Recombinant human Hif-1 alpha (1-85) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Protein Length: 1-85 a.a.
• Description: Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1 beta subunit and one of three subunits (Hif-1 alpha, Hif-2 alpha or Hif-3 alpha). The activation of Hif-1 alpha is closely associated with a variety of tumors and oncogenic pathways. Hif-1 alpha consists of DNA binding domain(DBD domain), dimerization domain and C-terminal regulatory domains, including two transactivation domains(TAD), an oxygen-dependent degradation(ODD) domain, and inhibitory domains.
• Concentration: 0.25 mg/ml
• Form: Supplied as a liquid in 20mM Tris buffer, pH 8.0, containing 20% glycerol, 1mM DTT, 0.2M NaCl and 1mM EDTA.
• Purity: > 80% by SDS - PAGE
• Sequence: 1-85 amino acids: MGSSHHHHHH SSGLVPRGSH MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV SSHLDKASVM RLTISYLRVR KLLDAGDLDI EDDMK
• Molecular Mass: 27.7 kDa (248aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: HIF1A hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) [ Homo sapiens ]
• Official Symbol: HIF1A
• Synonyms: HIF1A; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA; Hypoxia-inducible factor 1 alpha; HIF1 alpha; ARNT-interacting protein; Member of PAS protein 1; Basic-helix-loop-helix-PAS protein; ARNT interacting protein; hypoxia-inducible factor 1 alpha isoform I.3; member of PAS superfamily 1; hypoxia-inducible factor 1, alpha subunit
• Gene ID: 3091
• mRNA Refseq: NM_001530
• Protein Refseq: NP_001521
• MIM: 603348
• UniProt ID: Q16665
• Chromosome Location: 14q21-q24
• Pathway: Pathways in cancer; Renal cell carcinoma; mTOR signaling pathway
• Function: Hp90 protein binding; RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; protein heterodimerization activity; sequence-specific DNA binding; signal transducer activity; transcription factor binding