Recombinant Human Interleukin-12

• Cat.No.: IL12-21H
• Product Overview: Recombinant human interleukin 12 is a heterodimeric cytokine composed of covalently disulfide linked a light chain of p35 (P35, IL-12α) and a heavy chain of p40 (P40, IL-12β) with PI 4.5-5.5. Specifically, the heavy chain (P40) is consisted of 306 amino acids, while the light chain (P35) compounds 197 amino acids. It is approximately 75 kDa, containing 503 amino acids, non-glycosylated.

Specification

• Cat. No.: IL12-21H
• Description: Inerleukin-12 (IL-12), as a potent factor, plays an important role in the networks of immune system. It is a multifunctional cytokine, the properties of which bridge innate and adaptive immunity, acting as a key regulator of cell-mediated immune responses through the induction of T helper 1differentiation. By promoting IFN-γ production, proliferation, and cytolytic activity of natural killer and T cells, IL-12 induces cellular immunity. IL-12 not only can facilitate the maturation of antigen presenting cells (APC), especially for dendritic cells (DC), but also can be the third signal to change properties of CD8 T cells and increase generation of effector and memory cells. Treatment with IL-12 can inhibit bacterial, virus and intracellular parasites, produce an effect of anti-tumor, and enhance autoimmunity.
• Purity: Greater than 98% by SDS-PAGE gel and HPLC analyses.
• Specification: 10μg/bottle. Any other specifications, please feel free to contact us.
• Formulation: Sterile filtered through 0.2 micron filter, added the human serum albumin and mannitol and then lyophilized from 10mMPBS, pH=7.2.
• Specific Activity: the ED₅₀was determined by the stimulation of IFN-γ production by human peripheral blood mononuclear cells co-stimulated with IL-12 is ≤ 5ng/ml, corresponding to a specific activity of ≥ 8000 units/μg.
• Endotoxin: Endotoxin level is less than 1 EU/μg.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 day or -20°C for future use.
• Storage: The lyophilized is stable for 3 months at room temperature, can be stored for 2 years at 4°C , but best stored at -20°C . Reconstituted IL-12 should be stored in working aliquots at -20°C .
• Publication:
  Cytokine and reactivity profiles in SLE patients following anti-CD19 CART therapy (2023)

Gene Information

• Gene Name: IL12A
• Synonyms: interleukin12A(natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); P35; CLMF; NFSK; NKSF1; IL-12A; natural killer cell stimulatory factor 1, 35 kD subunit; cytotoxic lymphocyte maturation factor 1, p35; interleukin 12 p35;IL-12 subunit p35;NF cell stimulatory factor chain 1; interleukin-12 alpha chain; IL12A_HUMAN; Interleukin-12 subunit alpha [Precursor];IL-12 subunit p35;Cytotoxic lymphocyte maturation factor 35 kDa subunit;CLMF p35; IL12A
• mRNA Refseq: NM_000882
• Protein Refseq: NP_000873
• MIM: 161560
• UniProt ID: P29459
• Chromosome Location: 3q25.33-q26
• Gene ID: 3593
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus.
• Function: Contributes to cytokine activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; protein binding; protein heterodimerization activity.