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Recombinant Human Interleukin-12

Cat.No. : IL12-21H
Product Overview : Recombinant human interleukin 12 is a heterodimeric cytokine composed of covalently disulfide linked a light chain of p35 (P35, IL-12α) and a heavy chain of p40 (P40, IL-12β) with PI 4.5-5.5. Specifically, the heavy chain (P40) is consisted of 306 amino acids, while the light chain (P35) compounds 197 amino acids. It is approximately 75 kDa, containing 503 amino acids, non-glycosylated.
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Cat. No. : IL12-21H
Description : Inerleukin-12 (IL-12), as a potent factor, plays an important role in the networks of immune system. It is a multifunctional cytokine, the properties of which bridge innate and adaptive immunity, acting as a key regulator of cell-mediated immune responses through the induction of T helper 1differentiation. By promoting IFN-γ production, proliferation, and cytolytic activity of natural killer and T cells, IL-12 induces cellular immunity. IL-12 not only can facilitate the maturation of antigen presenting cells (APC), especially for dendritic cells (DC), but also can be the third signal to change properties of CD8 T cells and increase generation of effector and memory cells. Treatment with IL-12 can inhibit bacterial, virus and intracellular parasites, produce an effect of anti-tumor, and enhance autoimmunity.
Purity : Greater than 98% by SDS-PAGE gel and HPLC analyses.
Specification : 10μg/bottle. Any other specifications, please feel free to contact us.
Formulation : Sterile filtered through 0.2 micron filter, added the human serum albumin and mannitol and then lyophilized from 10mMPBS, pH=7.2.
Specific Activity : the ED50was determined by the stimulation of IFN-γ production by human peripheral blood mononuclear cells co-stimulated with IL-12 is ≤ 5ng/ml, corresponding to a specific activity of ≥ 8000 units/μg.
Endotoxin : Endotoxin level is less than 1 EU/μg.
Reconstitution : Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 day or -20°C for future use.
Storage : The lyophilized is stable for 3 months at room temperature, can be stored for 2 years at 4°C , but best stored at -20°C . Reconstituted IL-12 should be stored in working aliquots at -20°C .
Publication :
Cytokine and reactivity profiles in SLE patients following anti-CD19 CART therapy (2023)
Gene Name : IL12A
Synonyms : interleukin12A(natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); P35; CLMF; NFSK; NKSF1; IL-12A; natural killer cell stimulatory factor 1, 35 kD subunit; cytotoxic lymphocyte maturation factor 1, p35; interleukin 12 p35;IL-12 subunit p35;NF cell stimulatory factor chain 1; interleukin-12 alpha chain; IL12A_HUMAN; Interleukin-12 subunit alpha [Precursor];IL-12 subunit p35;Cytotoxic lymphocyte maturation factor 35 kDa subunit;CLMF p35; IL12A
mRNA Refseq : NM_000882
Protein Refseq : NP_000873
MIM : 161560
UniProt ID : P29459
Chromosome Location : 3q25.33-q26
Gene ID : 3593
Pathway : Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus.
Function : Contributes to cytokine activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; protein binding; protein heterodimerization activity.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How do mass spectrometry-based techniques reveal post-translational modifications on IL12A? 08/18/2022

Mass spectrometry identifies IL12A's post-translational modifications and their roles.

What strategies validate IL12A's role in immune response modulation using knockout models? 06/12/2022

Knockout models validate IL12A's roles in immune response modulation.

How do protein footprinting assays unveil IL12A's binding interfaces with receptor partners? 06/26/2021

Protein footprinting assays reveal IL12A's binding interfaces with receptors.

What experimental methods quantify IL12A's secretion kinetics and stability in cell culture? 12/17/2020

ELISA and stability assays quantify IL12A secretion and stability in culture.

Can you discuss the use of SPR in characterizing IL12A's interactions with antibodies and inhibitors? 10/25/2020

SPR characterizes IL12A's interactions with antibodies and inhibitors.

What cellular assays assess IL12A's ability to induce cytokine production in immune cells? 05/19/2019

Cellular assays gauge IL12A's cytokine induction in immune cells.

How does isothermal titration calorimetry provide insights into IL12A's binding affinity with receptors? 06/05/2018

Isothermal titration calorimetry measures IL12A's binding affinity with receptors.

What techniques evaluate the impact of IL12A genetic variants on protein structure and function? 03/08/2018

Structural biology techniques analyze IL12A genetic variant impacts.

Could you elaborate on the use of site-directed mutagenesis in probing IL12A's functional domains? 11/23/2017

Site-directed mutagenesis probes IL12A's functional domains and interactions.

How does in vivo bioluminescence imaging track IL12A expression dynamics in living organisms? 02/16/2017

Bioluminescence imaging tracks IL12A expression dynamics in vivo.

Customer Reviews (3)

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Reviews
01/14/2021

    Suitable for co-immunoprecipitation.

    12/21/2020

      Consistent in bioactivity.

      11/16/2020

        Good for receptor binding studies.

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