Active Recombinant Rat ApoA1

• Cat.No.: ApoA1-8446R
• Product Overview: Recombinant rat ApoA-I is a 27.4 kDa protein of 235 amino acid residues and was expressed in E.coli..

Specification

• Description: ApoA-I is a 29.0 kDa protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle. ApoA-I, which is found exclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This apoA-I ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is though to inhibit atherogenesis. For this reason HDL is also known as the “good cholesterol.” The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I (called apoA-I Milano). The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic Vascular diseases.
• Source: E. coli
• Species: Rat
• Form: Sterile filtered and lyophilized with no additives
• Bio-activity: Test in process
• Molecular Mass: 27.4 kDa
• Endotoxin: <0.1>
• Purity: ≥98% by SDS - PAGE; ≥98% by HPLC
• Storage: The lyophilized ApoA-I is best-stored desiccated below 0°C. Reconstituted ApoA-I should be stored at working aliquots at -20°C.
• Reconstitution: Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.
• Shipping: Blue ice

Gene Information

• Gene Name: Apoa1 apolipoprotein A-I [ Rattus norvegicus ]
• Official Symbol: ApoA1
• Synonyms: APOA1; apolipoprotein A-I; apo-AI; apolipoprotein A1; apolipoprotein A-1; preproapolipoprotein A-I; apoA-I
• Gene ID: 25081
• mRNA Refseq: NM_012738
• Protein Refseq: NP_036870
• Pathway: African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; PPAR signaling pathway, organism-specific biosystem; PPAR signaling pathway, conserved biosystem; Statin Pathway, organism-specific biosystem
• Function: apolipoprotein A-I receptor binding; apolipoprotein receptor binding; beta-amyloid binding; cholesterol binding; cholesterol transporter activity; cholesterol transporter activity; contributes_to cholesterol transporter activity; enzyme binding; high-density lipoprotein particle binding; high-density lipoprotein particle receptor binding; identical protein binding; lipase inhibitor activity; lipid binding; lipid binding; lipid transporter activity; lipoprotein particle binding; phosphatidylcholine-sterol O-acyltransferase activator activity; phospholipid binding; phospholipid transporter activity